LncRNA-MIAT在肿瘤坏死因子α介导的血管内皮细胞炎症中的表达及作用  

Expression and Effect of Lnc RNA-MIAT in Tumor Necrosis Factor-α Induced Endothelial Cell Inflammation

作　　者：任成龙[1] 张璐[2] 宁险峰[1] 赵青[1] 蔡尚郎[1] 张文忠[1] 

机构地区：[1]青岛大学附属医院心内科,山东省青岛市266003 [2]青岛大学附属医院中心实验室,山东省青岛市266003

出　　处：《中国循环杂志》2017年第6期607-611,共5页Chinese Circulation Journal

基　　金：山东省自然科学基金培养基金项目(ZR2014HP017)

摘　　要：目的:体外观察血管内皮细胞(ECs)在肿瘤坏死因子α(TNF-α)刺激下,细胞内长链非编码核糖核酸(LncR NA)心肌梗死相关转录本(LncR NA-MIAT)的表达变化,并探讨LncR NA-MIAT对ECs炎症的调控作用。方法:以TNF-α诱导ECs表达LncR NA-MIAT,采用蛋白质免疫印迹法(Western Blot)、聚合酶链反应(PCR)及实时荧光定量PCR(qR T-PCR)方法分别检测ECs炎症状态下细胞间黏附分子-1(ICAM-1)和LncR NA-MIAT的表达情况。应用MIAT小干扰核糖核酸(siR NAMIAT)转染ECs细胞,观察LncR NA-MIAT敲低对ICAM-1表达的影响。结果:Lnc RNA-MIAT随着TNF-α刺激时间的延长与浓度的增高呈上升趋势,刺激24 h、48 h分别与刺激0 h、6 h、12 h比较,Lnc RNA-MIAT表达量均增多,差异均具有统计学意义(P<0.05);TNF-α刺激浓度为1.000 ng/ml、10.000 ng/ml分别与刺激浓度为0.000 ng/ml、0.125 ng/ml比较,LncR NA-MIAT表达量增多,差异均具有统计学意义(P均<0.05)。ECs经siR NAMIAT转染后,TNF-α诱导的ICAM-1蛋白表达被显著抑制(P<0.05)。结论:LncR NA-MIAT可能参与血管ECs的炎症反应,并可能起到促炎作用。Objective： To observe the expression of long non-coding RNA myocardial infarction associated transcript （LncRNA-MIAT） in tumor necrosis factor-α （TNF-α） induced endothelial cells （ECs） inflammation in vitroand to study the impact of LncRNA-MIAT on inflammatory regulation. Methods： LncRNA-MIAT expression in ECs was induced by TNF-α at different time and concentration. Expressions of intercellular adhesion molecule-1 （ICAM-1） and LncRNA-MIAT in inflammatory ECs were examined by quantitative real time polymerase chain reaction （qRT-PCR） and Western blot analysis. Moreover, ECs was transfected by siRNAMIAT to observe the effect of LncRNA-MIAT knock-down on ICAM-1 expression. Results： LncRNA-MIAT expression showed the increasing trend by elevated time and concentration of TNF-stimulation. Compared with TNF-α stimulation at 0h, 6h and 12h, LncRNA-MIAT expressions were increased at 24h and 48h of TNF-αstimulation respectively, allP〈0.05; compared with TNF-α concentration at 0ng/ml and 0.125ng/ml, LncRNA-MIAT expressions were elevated by TNF-α stimulation at 1.000ng/ml and 10.000ng/ml respectively, allP〈0.05. With siRNAMIAT knock-down, TNF-α induced ICAM-1 protein expression was significantly reduced in ECs,P〈0.05. Conclusion： LncRNA-MIAT might be involved in ECs inflammatory response and it may play a role to promote inflammation.

关 键 词：核糖核酸酶类 肿瘤坏死因子Α 细胞间黏附分子-1 

分 类 号：R54[医药卫生—心血管疾病]