乌梅提取物对纳米细菌致大鼠肾损伤的修复与拮抗结石生成的关系研究  

Effect of Fructus Mume Extract on Repair of Renal Injury Induced by Nanobacteria in Rats and Its Relationship with Antagonistic Stone Formation

作　　者：夏剑锋[1] 谷江[1] 张永春[1] 刘淼[1] 董安涛 杨锦春[1] 

机构地区：[1]贵州医科大学附属医院泌尿外科,贵阳550004

出　　处：《中国医科大学学报》2017年第7期640-644,共5页Journal of China Medical University

基　　金：贵阳市科技计划(2014100145)

摘　　要：目的构建纳米细菌(NB)致肾结石大鼠模型,观察乌梅提取物对早期肾损伤分子1(KIM-1)和骨桥蛋白(OPN)的影响,分析乌梅提取物对早期肾结石的治疗意义。方法从人上尿路结石分离并培养NB。将54只雄性SD大鼠随机分为空白组、诱石组和治疗组,采取尾静脉注射NB构建肾结石模型,以乌梅提取物干预模型,并在不同时期分批处死大鼠,实时PCR检测肾组织KIM-1 m RNA的表达,ELISA检测尿KIM-1浓度,HE染色观察肾组织结石晶体情况,并用免疫组化方法检测OPN表达。结果诱石组及治疗组肾结石模型大鼠出现肾小管扩张,肾小管结石晶体形成,早期KIM-1和OPN的表达升高,上述变化与NB的注射时间呈正相关,乌梅提取物可拮抗该类变化。结论乌梅提取物可能通过修复肾损伤减少肾结石形成,该机制可能与乌梅提取物对KIM-1、OPN的基因调节有关。Objective To observe the early effect of Fructus mume extract on KIM- 1 and OPN levels in rats with kidney stone formation induced by nanobacteria and to investigate the therapeutic significance of F. mume extract on early kidney stone formation. Methods Nanobacteria were separated and cultured from human upper urinary calculi. The study group appropriately included 54 healthy male SD rats. The renal calculus model was constructed by tail vein injection of nanobacteria. A kidney stone model was created with an F. mume extract intervention, and rats were killed at different stages of the intervention. Real-time polymerase chain reaction was used to detect the KIM- 1 mRNA expression in rat renal tissue, and the KIM- 1 concentration in urine was detected by using enzyme-linked immunosorbent assay. We detected kidney tissue stone crystals and OPN expression by using hematoxylin-eosin staining and immunohistochemistry. Results Renal tubules of the experimental modal were signiiicanlly expanded,that is,the fomation of renal tubular stones. The early KIM-1 and OPN expression levels were increased. The above-mentioned changes positively con＇elated with the injection time of the nanobacteria, and F. mume extract antagonized the changes. Conclusion F. mume extract may be useful for the repair of renal injury to reduce kidney stone formation,which may be related to the gene regulation of KIM- 1 and OPN.

关 键 词：乌梅提取物 纳米细菌 肾结石 损伤修复 

分 类 号：R692.4[医药卫生—泌尿科学]