番茄泛素活化酶基因家族进化及表达模式分析  

Identification and Expression Analysis of the Ubiquitin Activating Enzyme Gene Family in Tomato

作　　者：赵秋芳[1,2] 马海洋[1,2] 陈曙[1,2] 陈宏良[1,2] 贾利强[1,2] 

机构地区：[1]中国热带农业科学院南亚热带作物研究所,广东湛江524091 [2]农业部热带果树生物学重点实验室,广东湛江524091

出　　处：《热带作物学报》2017年第6期1081-1087,共7页Chinese Journal of Tropical Crops

基　　金：中央级公益性科研院所基本科研业务专项(No.1630062014010;1630062015017)

摘　　要：泛素活化酶是蛋白泛素化所需的第一个酶,在泛素蛋白酶体途径中发挥重要作用。本文利用生物信息学方法从番茄基因组中鉴定出2个泛素活化酶(ubiquitin activating enzyme,UBA)基因,命名为Sl UBA1和Sl UBA2。序列分析表明Sl UBA1和Sl UBA2基因的CDS序列长分别为3 060和3 255 bp,分别编码1 019和1 084个氨基酸,编码蛋白分子量为114.15和120.46 ku,分别位于第6和9染色体。2个基因均含有5个内含子,编码蛋白均为酸性、疏水性蛋白;对蛋白二级结构分析发现2个UBA蛋白均以α-螺旋和无规则卷曲为主;亚细胞定位预测均定位于细胞核。序列比对和进化树分析表明番茄UBA基因与其他物种UBA基因相似性高,进化过程非常保守。番茄不同组织表达分析结果表明2个UBA基因在根系和花的表达量较高,果实成熟过程中的表达量相对较低。非生物胁迫试验结果表明:Sl UBA1基因在低温、干旱和盐胁迫下均上调表达;而Sl UBA2基因对非生物胁迫没有明显响应,这些结果表明Sl UBA1基因可能参与番茄对低温、干旱和盐胁迫的响应。Ubiquitin activating enzyme is the first enzyme which combined with ubiquitin and protein, playing an important role in the ubiquitin-proteasome pathway（ UPP）. In this study, we identified two ubiquitin activating enzyme（ UBA） genes designated as Sl UBA1 and Sl UBA2 from tomato genome using bioinformatics methods.Sequence analysis of Sl UBA1 and Sl UBA2 revealed that the CDS lengths waere 3 060 and 3 255 bp respectively,encoding the proteins of 1 019 and 1 084 amino acids with predicted molecular weight of 114.15 and 120.46 ku,located on No.6 and 9 chromosome, separately. The two Sl UBA genes both contained 5 introns, encoding acidic and hydrophobic protein. The predicted secondary structure suggested that the main structure of two proteins were alpha helix and random coil; The subcellular localization were predicted in the nucleus. Sequence alignment and phylogenetic tree analysis showed that the UBA genes of tomato had high similarity to the UBA genes in other species and indicated that the evolution process of the UBA family was very conservative. The expression of two Sl UBA genes in different tissues were investigated, which were highly expressed in roots and flowers, while had low expression in fruit ripening stage. Abiotic stress treated results showed that the expression levels of Sl UBA1 was upregulated under cold, drought and salt stress, while the gene of Sl UBA2 had no significant change. These results indicated that Sl UBA1 genes may be involved in the response of tomato to low temperature, drought and salt stresses.

关 键 词：番茄 泛素活化酶基因 进化分析 基因表达 

分 类 号：S641.2[农业科学—蔬菜学]