野生红果枸杞白粉病菌的鉴定  

Powdery Mildew Authentication of Lycium chinense

作　　者：马淑雅[1,2] 徐克东[1,2] 吴建新[1,2] 王伟[1,2] 杨灿 李成伟[1,2,3] 

机构地区：[1]周口师范学院植物遗传与分子育种重点实验室,河南周口466001 [2]河南省作物分子育种与生物反应器重点实验室,河南周口466001 [3]河南科技学院生命科技学院,河南新乡453003

出　　处：《贵州农业科学》2017年第6期79-83,共5页Guizhou Agricultural Sciences

基　　金：国家自然科学基金项目"病原物效应子及与其互作番茄靶标基因的发掘和功能分析"(31272168);河南省科技厅项目"人干扰素-γ的细胞工程化生产研究"(144300510064);河南省教育厅项目"RcAGL15和RcFUS3启动狗蔷薇类原球茎胚萌机制的研究"(14A180003)

摘　　要：为白粉病菌的系统进化、致病机理及防控深入研究提供基础资料和基因资源,采用形态学、分子生物学和科赫法则相结合的方法对河南省周口地区野生红果枸杞白粉病的致病病原菌生理小种类群进行研究。结果表明:该致病菌分生孢子呈长椭圆形,大小为(12.5~16)μL×(17.5~21.5)μL。其克隆获得目的序列长度为548 bp(GenBank:KX579506.1),与已报道的穆氏节丝壳属白粉病菌JX546296.1、AF073358.1、AF455748.1和AB022380.1的ITS序列同源率分别为86.39%、87.21%、86.68%和80.19%。初步确定该病原菌属穆氏节丝壳属。The morphological statistics, molecular biological identification, and Koch postulates were synthetically used for the pathogen authentication of powdery mildew on L. chinense in Zhoukou, Henan province, so as to provide basic data and genetic resources for system evolution, pathogenesis, prevention and control of powdery mildew. Results： Conidium of powdery mildew was oblong, the scale was （12.5- 16）μL× （17.5-21.5）μL. The isolated sequence was amplified with specific primers on ribosomal DNA with length of 548 bp（GenBank： KX579506. 1）. The homologous rates of the isolated sequence among many reported sequences of Arthrocladiella mougeotii, such as JX546296.1, AF073358. 1, AF455748. 1 and AB022380.1, were 86.39%, 87.21%, 86.68% and 80. 19%, respectively. The results indicated that the powdery mildew infected on L. chinense was A. mougeotii.

关 键 词：枸杞 白粉病菌 科赫法则 核糖体DNA转录间隔区 穆氏节丝壳属 

分 类 号：S435.671[农业科学—农业昆虫与害虫防治]