白念珠菌氟康唑耐药株与敏感株SRB1、CDR1、ERG11表达比较分析  

Analysis of expression of SRB1、CDR1、ERG11 in fluconazole sensitive and resistant Candida albicans strains

作　　者：高来强 王海英 王学红 李爱兰 马强 张宏[2] 

机构地区：[1]东营市人民医院皮肤科,东营257091 [2]暨南大学真菌病研究所,广州510632

出　　处：《中国真菌学杂志》2017年第3期148-151,155,共5页Chinese Journal of Mycology

基　　金：山东省自然科学基金项目(ZR2015PH061)

摘　　要：目的了解SRB1在白念珠菌氟康唑耐药株和敏感株表达差异,探讨与白念珠菌耐药性的关系。方法使用同一亲本来源的白念珠菌氟康唑耐药株CA-16和敏感株CA-3为研究对象,采用实时荧光定量RT-PCR的方法扩增各菌株的目的基因SRB1、CDR1、ERG11,观察各菌株目的基因表达情况。结果与白念珠菌氟康唑敏感株CA-3相比,在mRNA水平氟康唑耐药株CA-16的SRB1和耐药基因CDR1、ERG11表达升高,SRB1、CDR1、ERG11表达水平差异具有统计学意义(P<0.05)。结论白念珠菌SRB1的表达增高和白念珠菌对氟康唑耐药密切相关,SRB1可能是一个新的耐药候选基因。Objective To investigate the differences in the expression of Candida albicans SRB1 in Candida albicans flu- conazol resistant strain and sensitive strain,and to explore the relationship between SRB1 and drug resistance in Candida al- bicans.Methods Two Candida albicans strains were from a same parent.We used real-time RT-PCR to observe the expres- sion of the target genes of SRB1 ,CDR1 ,ERG11 in Candida albicans fluconazole resistant strain CA-16 and sensitive strain CA-3.Result Compared with CA-3,the expression of the mRNA of SRB1 and drug resistance gene CDR1 ,ERG11 in CA 16 increased.There was significant difference in the expression levels of SRR1 .CDR1 and ERG11 （P〈0.05）.Conclusion The increased expression of SRB1 and the Candida albicans resistance to fluconazole was closely related, and SRB1 might be a new candidate genes associated with drug resistance.

关 键 词：白念珠菌 耐药 基因 PCR SRB1 

分 类 号：R379.4[医药卫生—病原生物学]