HLA-DQA1基因拷贝数变异与乙型肝炎病毒感染后不同转归的易感性  

Association between the copy number variation of HLA-DQA1 gene and the susceptibility to the outcome of HBV infection

作　　者：李卫[1] 郜玉峰[2] 饶建国[3] 李旭[2] 

机构地区：[1]安徽医科大学阜阳传染病临床学院(阜阳市第二人民医院)肝病科,阜阳236000 [2]安徽医科大学第二附属医院肝病科,合肥230601 [3]六安市人民医院感染病科,六安237000

出　　处：《安徽医科大学学报》2017年第7期1053-1056,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81273142)

摘　　要：目的探讨人类白细胞抗原(HLA)DQAl基因拷贝数变异(CNV)与中国汉族人群乙型肝炎病毒(HBV)感染后不同转归和疾病进展的关系。方法采用AccuC opy CNV检测技术检测825例慢性HBV感染患者和287例急性自限性HBV感染者的HLA-DQAl基因CNV,采用χ2检验等分析HLA-DQA1 CNV与HBV感染后慢性化和疾病进展的发病风险。结果在HBV感染后急慢性转归方面,急性自限性HBV感染组HLA-DQA1拷贝数>2的比例显著高于慢性HBV感染组(15.3%vs 6.9%,χ2=25.22,P<0.001)。在慢性HBV感染后疾病进展方面,随疾病进展,HLA-DQAl基因拷贝数<2的比例在慢性乙型肝炎(CHB)组、肝硬化(LC)组和肝癌(HCC)组3组间逐渐增加,分别为10.1%,15.3%和27.9%,差异有统计学意义(χ2=25.66,P<0.001)。根据E抗原分组分析显示,E抗原阳性患者组和E抗原阴性患者组HLA-DQA1 CNV的比例无显著性差异。结论 HLA-DQA1基因CNV是HBV感染慢性化的遗传易感因素,HLA-DQA1基因拷贝数减少可能是HBV感染慢性化和疾病进展的危险因素。Objective To explore the association between the copy number variation （CNV） of HLA-DQA1 gene and the outcome of HBV infection in Chinese Han population. Methods The HLA-DQA1 CNV of 825 patients with chronic HBV infection and 287 patients with acute self-limiting HBV infection was detected by AccuCopy assay. The chi-square test was used to evaluate the impact of HLA-DQA1 CNV on the outcome and disease progression after HBV infection. Results In the outcome of HBV infection, the proportion of HLA-DQA1 〉2 copy number in acute self-limiting HBV infection group was significantly higher than the proportion in chronic HBV infection group, 15.3% vs 6.9% ,P 〈0. 001. In the disease progression of chronic HBV infection, the proportion of HLA-DQA1 〉 2 copy number in the CHB, LC and HCC groups gradually increased, 10. 1%, 15.3%, 27.9%, P 〈0.001. There was no significant difference between the proportion of the HLA-DQA1 copy number in the E antigen positive patients group and E antigen negative patients. Conclusion The CNV of HLA-DQA1 gene is the susceptibility factor of chronic HBV infection and the decrease of HLA-DQA1 copy number is a risk factor for the ehronieity of HBV infection and disease progression after chronic HBV infection.

关 键 词：乙型肝炎病毒 人类白细胞相关抗原DQA1 拷贝数变异 基因易感性 

分 类 号：R512.62[医药卫生—内科学] R394.5[医药卫生—临床医学]