纹状体神经元在运动疲劳中枢调控中的区域特征分析  

Analysis of Regional Characteristics of Striatal Neurons Activities in Rats with Exercise-induced Fatigue

作　　者：侯莉娟[1] 程明晨 刘晓莉[1] 张吉敏[1] 乔德才[1] 

机构地区：[1]北京师范大学体育与运动学院,北京100875

出　　处：《中国运动医学杂志》2017年第6期486-492,共7页Chinese Journal of Sports Medicine

基　　金：国家自然科学基金(31171138/31401018);北京市自然科学基金(5142012)

摘　　要：目的:观察运动疲劳后大鼠纹状体背内侧部和背外侧部神经元电活动的变化,探寻纹状体神经元在运动疲劳中枢调控中的区域特征。方法:筛选出36只雄性Wistar大鼠随机分为对照组(CG组)、一次性力竭运动组(SF组)和重复力竭运动疲劳组(RF组),每组各12只。疲劳模型建立采用递增负荷运动方案,CG组大鼠不运动,SF组大鼠进行一次力竭运动,RF组大鼠每天进行一次力竭运动,连续6天;各组大鼠建模结束后即刻采用玻璃微电极胞外技术分别记录纹状体背内侧部(n=6)和背外侧部(n=6)神经元自发电活动。采集到的电信号主要选择放电频率、放电特征及不同类型神经元放电频率等指标进行分析。结果:(1)一次性力竭运动疲劳后纹状体神经元放电频率显著高于对照组(P<0.05),而背外侧部重复力竭运动疲劳后较一次性力竭运动后明显降低(P<0.01);(2)重复力竭运动后纹状体神经元不规则放电比例较对照组降低(P<0.05),爆发放电显著增加(P<0.05);(3)运动疲劳后纹状体背内侧部中等多棘神经元(MSNs)放电频率显著增高(P<0.05),且重复力竭运动疲劳后显著高于背外侧部(P<0.01),快放电中间神经元(FS)在重复力竭运动疲劳后放电频率均明显降低,一次性力竭组大鼠背外侧大型胆碱能神经元(LANs)放电频率较对照组明显降低(P<0.05),而重复力竭组较一次性力竭组显著升高(P<0.05)。结论:(1)纹状体神经元在参与运动疲劳中枢调控中呈现区域特征,且在疲劳发生和积累的不同时段作用有所不同,这与纹状体背内侧部和背外侧部神经元分别接受不同类型投射神经元有关;(2)纹状体快放电中间神经元在运动疲劳后神经元电活动的剂量效应关系中起重要的调节作用。Objective To observe the local field potential activity of dorsomedial and dorsolater striatal（STR）neurons in rats with exercise-induced fatigue,so as to explore the regional characteristics ofthose neurons. Methods Thirty-six male Wistar rats were randomly divided into a control group（CG）,asingle fatigue group（SFG）and a repeated fatigue group（RFG）,each of 12. The exercise-induced fatiguemodel was established according to a load-increasing treadmill running protocol. The CG did not doany treadmill running,SFG finished one-time exhaustive exercise while the RFG repeated exhaustive ex-ercise 6 times. The extracellular glass microelectrode technique was used to record the spontaneous firing of dorsomedial（n=6）and dorsolateral（n=6）STR neurons in vivo to observe the discharging frequency,characteristics and types. Results（1）Spontaneous firing frequencies of striatal neurons in SFG were significantly higher than those of CG（P〈0.01）,while those of RFG were significantly lower than SFG.（2）After repeated fatigues,there were significant decreases in the irregular firing pattern and significant increase in the explosive firing of striatal neurons compared with CG（P〈0.05）.（3）After the single fatigue and repeated fatigue,the discharging frequency of dorsomedial medium spiny-like neurons increased significantly（P〈0.05）,and that after repeated fatigue significantly higher than that of the dorso-lateral（P〈0.01）. The discharge frequency of the dorsomedial and dorsolateral fast-spiking neurons decreased significantly after repeated fatigues. The discharge frequency of dorsolateral large aspiny-likeneurons of RFG was significantly higher than SFG,while that of the latter was significantly lower thanCG（P〈0.05）. Conclusions（1）Striatal neurons mediate exercise-induced fatigue and present regionalcharacteristics,which might be due to dorsomedial and dorsolateral striatal neurons receiving differenttypes of projection neurons.（2）Striatal fast-spiking neurons may p

关 键 词：运动疲劳 大鼠 纹状体神经元 电活动 区域特征 

分 类 号：G804.7[文化科学—运动人体科学]