miR-1246和miR-1261在模拟失重48 h后人肺微血管内皮细胞中的表达及生物信息学分析  

Expression of miR-1246 and miR-1261 Induced by 48 h Simulated Weightlessness in Human Pulmonary Microvascular Endothelial Cells and Bioinformatics Analysis

作　　者：高原[1] 徐沁[2] 褚孟洋 张丽君[2] 李程飞[1] 石菲[1] 孙喜庆[1] 赵疆东[1] 闫铭[3] 王永春[1] 

机构地区：[1]第四军医大学航空航天医学系航空航天生物动力学教研室,陕西西安710032 [2]第四军医大学学员一旅,陕西西安710032 [3]第四军医大学西京医院骨科,陕西西安710032

出　　处：《贵州医科大学学报》2017年第6期639-645,共7页Journal of Guizhou Medical University

基　　金：国家自然科学基金项目(81372130;81301681;81301581;81471817)

摘　　要：目的:检测模拟失重48 h人肺微血管内皮细胞(HMVEC-L)miR-1246和miR-1261表达的改变,利用生物信息学方法分析预测其靶基因。方法:以回转器行模拟失重效应48 h的HMVEC-L为模拟失重组,同时设正常重力对照组,比较两组细胞miRNA表达谱的变化,应用Real-time PCR检测HMVEC-L细胞miR-1246和miR-1261的表达,通过Target Scan、miRDB和miRanda对miR-1246和miR-1261的靶基因进行预测以及功能注释和通路富集分析。结果:与正常重力对照组比较,模拟失重48 h可引起HMVEC-L细胞中29种miRNA呈现显著差异性表达,miR-1246和miR-1261显著降低,差异有统计学意义(P<0.05);生物信息学分析显示miR-1246和miR-1261的靶基因涉及生物过程、分子功能及细胞组成等多种功能,主要参与了磷酸化通路、凋亡相关通路和细胞骨架等相关分子的调控。结论:模拟失重48 h后可引起HMVEC-L细胞miR-1246和miR-1261的表达降低,提示其可能参与了模拟失重后内皮细胞功能异常的调控。Objective： To detect the changes of miR-1246 and miR-1261 expression after 48 h simulated weightlessness in human pulmonary microvascular endothelial cells,and further explore the predicted target gene by bioinformatics analysis. Methods： The clinostat was employed for simulated weightlessness,HMVEC-L underwent 48 h as simulated weightless group,and normal gravity group was set as control group. Comparing changes of miRNAs,real-time PCR was applied to confirm the expression of miR-1246 and miR-1261 obtained by microarray analysis. The possible target genes of miR-1246 and miR-1261 were predicted,functional annotated,pathway enrichment analyzed by TargetS can,miRDB and miRanda. Results： Compared with the control group,48 h simulated weightlesscould induce 29 miRNAs expressions with significant changes in HMVEC-L（ P〈0. 05）; the expression of miR-1246 and miR-1261 decreased significantly（ P〈0. 05）. The predicted miR-1246 and miR-1261 target genes by bioinformatics analysis were involved in biological process,molecular function and cellular composition,which composed the molecular regulation of phosphorylation pathway,apoptosis pathway and cytoskeleton. Conclusion： The expression of miR-1246 and miR-1261 was obviously decreased in HMVEC-L induced by 48 h simulated weightlessness. Bioinformatics analysis showed the changes of miR-1246 and miR-1261 expression might involve in the regulation of dysfunction of endothelial cells induced by simulated weightlessness.

关 键 词：肺 模拟失重 血管内皮细胞 miR-1246 miR-1261 生物信息学 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]