花生MAPK基因的筛选和盐胁迫分析  

Screening and Salinity Stress Analysis of MAPK Genes in Peanut

作　　者：孔祥远[1] 陈冠旭[1] 秦贵龙 隋炯明[1] 乔利仙[1] 王晶珊[1] 徐丽丽[1] 

机构地区：[1]青岛农业大学生命科学学院山东省高校植物生物技术重点实验室,山东青岛266109

出　　处：《华北农学报》2017年第3期27-32,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家自然基金项目(31571705;31301356;31471542);山东省科技发展计划项目(2014GNC110002)

摘　　要：促有丝分裂原活化蛋白激酶(MAPK)在植物生长发育及抗逆胁迫过程中发挥重要作用。为了解花生中MAPK基因的情况,利用RNA测序技术对花生耐盐突变体(S2)和对照(S4)进行了转录组分析,筛选出了14个MAPK基因,位于花生野生种基因组A组的6条染色体上。聚类分析表明,花生14个MAPK基因中13个可以聚到已报道的4个亚类中。利用花生S2和S4构建了盐胁迫处理前后的表达谱,根据调整后的P值,筛选出6个MAPK基因在S2和(或)S4受盐胁迫诱导表达,分别属于A(1个)和D亚类(5个)。为花生MAPK基因的功能验证和利用奠定了基础。Mitogen activated protein kinase （MAPK） plays an important role in plant growth and development,and stress resistance in plant.In order to improve our understanding of the MAPK genes in peanut,RNA-seq was used to analyze one transcriptomic library constructed using a mutant with higher salinity resistance （S2） and its control （S4） in this study,14 MAPK genes were screened and located on six chromosomes of A genome from wild species.Clustering analysis showed that 13 out of 14 MAPK genes were assorted to four subgroups reported previously.Digital gene expression profiles were constructed using S2 and S4 samples before and after salinity treatment,according to the adjusted P value,six MAPK genes were found to be responsive to salinity treatment in S2 and （or） S4,which belonged to A （1）and D （5） subgroups,respectively.This study can provide the basis for functional identification and application of MAPK genes in peanut.

关 键 词：花生 MAPK基因 盐胁迫 RNA测序 

分 类 号：Q78[生物学—分子生物学] S565.03[农业科学—作物学]