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作 者:魏杰[1] 王洪[1] 巩薇[1] 李晓波[1] 付瑞[1] 王吉[1] 邢进[1] 冯育芳[1] 王淑菁[1] 高正琴[1] 岳秉飞[1]
出 处:《实验动物科学》2017年第2期26-30,共5页Laboratory Animal Science
基 金:中国食品药品检定研究院中青年基金(No.2014C4)
摘 要:目的对北京地区两个封闭群巴马小型猪群体进行遗传质量评价与分析。方法利用DB11/T828.3-2011中的25对微卫星引物,对A、B两个巴马小型猪群体进行微卫星分型及遗传参数计算。结果 A、B两巴马小型猪群体分别得到126和160个等位基因,平均杂合度分别为0.6541、0.6973,平均多态信息含量(PIC)分别为0.6037、0.6605,两者的遗传距离为0.2650。结论 B群巴马小型猪群体具有较好的遗传多样性和稳定性,符合封闭群动物的群体遗传特征。Objective To analyze and evaluate the population genetic quality of 2 outbred BA-MA miniature pigs in Beijing. Method Twenty-five pairs of microsatellite primers were used in A group and B group outbred BA-MA miniature pigs according to the local standard DBI1/T828.3 -2011. And software Popgen32 was used to process the data. Result Twenty-five microsatellite loci shared 126 and 160 alleles in A group and B group outbred BA- MA miniature pigs, respectively. The average heterozygosity was 0. 6541 and 0. 6973, respectively, while the average polymorphism information content (PIC) was 0. 6037 and 0. 6605, respectively. The genetic distance between the two groups was 0. 2650. Conclusion B group BA-MA miniature pigs have adaptive genetic stability and genetic diversity, which satisfied with the genetic characteristics of closed colony laboratory animal.
关 键 词:巴马小型猪 遗传结构分析 DB11/T828.3-2011
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