正交设计优化拟缺香茶菜总黄酮提取工艺  

Optimization of the Extraction Technology of Total Flavonoids from Isodon excisoides by Orthogonal Experiment

作　　者：陈慧平[1] 李晓宁[2] 罗含希 邹敏[1] 

机构地区：[1]郑州大学医药科学研究院,河南郑州450052 [2]云南中医学院,云南昆明650500

出　　处：《中国现代中药》2017年第6期845-848,共4页Modern Chinese Medicine

基　　金：河南省自然科学基金(152300410159);河南省高等学校重点科研项目(16A350011)

摘　　要：目的:优化拟缺香茶菜总黄酮提取工艺。方法:采用L9(34)正交试验,以拟缺香茶菜总黄酮的含量为综合指标,采取加热回流方法,考察提取温度(A)、液料比(B)、提取时间(C)、乙醇浓度(D)4个因素对提取效果的影响。结果:最佳提取工艺为温度90℃、液料比40∶1、提取2.0 h、60%乙醇,平均得率为5.668 6%。结论:该工艺简便合理,稳定可行,可为拟缺香茶菜总黄酮提取工艺的确定提供依据。Objective：The optimal conditions of total flavonoids from Isodon excisoides were investigated by orthogonal array design.Methods：The content of total flavonoids was determined spectrometrically using rutin as the reference substance.The effects of extraction temperature,extraction time,liquid/material ratio,ethanol concentration on the content of total flavonoids in extracts were investigated by one-factor-at-a-time method,and the extraction conditions were optimized by orthogonal array design.Results：Ethanol concentration and liquid/material ratio were the key factors that affected the extraction of total flavonoids.The optimal extractive conditions were obtained as follows：temperature 90 ℃,treatment time 120 min,liquid/material ratio 50∶1 and ethanol concentration 50%.Under these conditions,the extraction yield of total flavonoids from I.excisoides was 5.668 6%.Conclusion：The optimized process remarkably increases the extraction yield of total flavonoids from I.excisoides and has a promising prospect for practical applications.

关 键 词：拟缺香茶菜 总黄酮 正交试验 提取工艺 

分 类 号：R282.71[医药卫生—中药学]