大豆MYB转录因子GmMYB010与其拟南芥同源基因的功能差异  

Characterization of different functions between a soybean MYB transcription factor GmMYB010 and its orthologous gene in Arabidopsis thaliana

作　　者：陆捡花 李祥[1] 赵为[1] 沈欣杰[1] 郭葳[1] 王笑一[1] 周新安[1] 矫永庆[1] 

机构地区：[1]中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062 [2]中国农业科学院研究生院,北京100081

出　　处：《中国油料作物学报》2017年第3期281-293,共13页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31371654和31522042);国家转基因重大专项(2016ZX08004003);湖北省自然科学基金(2016CFA049);中国农业科学院创新工程

摘　　要：MYB-related转录因子在植物生长发育和响应逆境胁迫过程中具有重要作用。本研究旨在分析大豆MYB转录因子GmMYB010与其在拟南芥中的同源基因AtMYB010的功能差异。序列分析表明,大豆的GmMYB010与拟南芥中AtMYB010都属于MYB-related转录因子家族中R-R-type类型;亚细胞定位结果表明两种蛋白均定位在核上;qRT-PCR检测结果表明,GmMYB010在大豆叶片中表达量最高,而AtMYB010在花中表达量最高;酵母检测发现,GmMYB010具有转录自激活活性,而AtMYB010不具有转录自激活活性。将GmMYB010与其拟南芥同源基因AtMYB010分别在野生型拟南芥中过量表达,表型观察发现GmMYB010过表达植株表型与野生型拟南芥一样,而AtMYB010过表达植株出现了矮小、多分枝等生长表型。对MAXs作用途径基因的检测发现,AtMYB010过量表达影响了独脚金内酯代谢途径,从而出现多分枝的表型。以上结果表明,GmMYB010和AtMYB010虽然是直系同源基因,但功能却发生了明显的分化。大豆GmMYB010在大豆中可能具有独特的功能,有待进一步研究。The MYB-related transcription factors play pivotal roles in regulating plant development and responses to diverse stresses. Functional differences between a MYB transcription factor GmMYB010 in soybean and its orthologous gene AtMYB010 in Arabidopsis thaliana was preliminary analysed in this study. The analysis of amino acid sequences showed that both GmMYB010 and AtMYB010 belonged to the family of R-R-type of MYB-related transcription factors. Subcellular localization exhibited that both GmMYB010 and AtMYB010 were located in the nucleus. The results of qRT-PCR indicated that GmMYB010 had the highest expression level in leaves,while AtMYB010 was expressed in a highest level in flowers. GmMYB010 showed strong transcriptional activating functions in yeast,while no transcriptional activating activity was found for AtMYB010. We transformed GmMYB010 gene and its orthologous gene AtMYB010 into Arabidopsis. The results showed that overexpssion of GmMYB010 in Arabidopsis did not cause any changes of phenotypes. However,overexpression of AtMYB010 led to dwarf and more branching phenotypes in transgenic Arabidopsis. The expression analyses of MAXs-related genes indicated that overexpression of AtMYB010 might have affected the Arabidopsis development through the strigolactone pathway. Taken together,our study showed that,although GmMYB010 and AtMYB010 were orthologous genes,they had apparently different functions. The specific function of GmMYB010 in soybean needed to be elucidated in futher research.

关 键 词：MYB-related GmMYB010 直系同源基因 模式植物 过表达 

分 类 号：S565.103[农业科学—作物学]