酶联免疫吸附法检测与甲苯胺红不加热血清试验在梅毒检验中应用价值的比较  

The Application Value Comparison of Enzyme-Linked Immunosorbent Assay and Toluidine Red Unheated Serum Test in the Detection of Syphilis

作　　者：肖登峰[1] 

机构地区：[1]湖北职业技术学院附属惠济医院检验科,武汉432000

出　　处：《生物医学工程学进展》2017年第2期110-111,118,共3页Progress in Biomedical Engineering

摘　　要：目的探讨酶联免疫吸附法(ELISA)检测与甲苯胺红不加热血清试验(TRUST)在梅毒检验中应用价值。方法回顾分析260例梅毒患者,均应用ELISA法与TRUST法检测血清梅毒螺旋体,比较梅毒螺旋体检出率,并分析其敏感性及特异性。结果 ELISA法对梅毒螺旋体检出率为95.8%(249/260),显著高于TRUST法64.6%(168/260),比较具有统计学差异(P<0.05);ELISA法诊断梅毒螺旋体的特异性为35.4%(92/260),阳性预测值为59.7%(249/417),阴性预测值为89.3%(92/103),准确率为65.6%(341/520)。结论 ELISA法对梅毒螺旋体检出率较高,可作为大样本梅毒筛查检验,值得临床选择。Objective To study the application value of enzyme-linked immunosorbent assay( ELISA)and toluidine red unheated serum test( TRUST) in the detection of syphilis. Methods A retrospective analysis on 260 cases of syphilis patients confirmed in our hospital was carried out,and both ELISA method and TRUST method were adopted to detect the patients' serum treponema pallidum. Then,the detection rates of treponema pallidum by the two methods were compared and the sensitivity and specificity were analyzed. Results The detection rate of treponema pallidum by ELISA method was 95. 8%( 249/260),which was significantly higher than the result of detected by TRUST 64. 6%( 168/260)( P < 0. 05). The specificity,the positive predictive value,the negative predictive value and the accuracy rate of ELISA method in the detection of treponema pallidum were 35.4%( 92/260),59. 7%( 249/417),89. 3%( 92/103) and 65. 6%( 341/520),respectively. Conclusion The detection rate of treponema pallidum by ELISA method is higher as compared to the TRUST. The ELISA method can be used for the syphilis screening test on a large number of samples and is worthy for clinical choice.

关 键 词：酶联免疫吸附法 甲苯胺红不加热血清法 梅毒 

分 类 号：R446.6[医药卫生—诊断学] R759.1[医药卫生—临床医学]