电针足三里穴对依托咪酯麻醉大鼠急性应激反应的影响  

Effect of electroacupuncture at tsusanli on regulation of stress response under etomidate anesthesia in rats

作　　者：蒋奕红[1] 胡越[1] 熊威威[1] 谭毅[1] 李爱国[1] 杨华[2] 黄志华[1] 

机构地区：[1]桂林医学院附属医院麻醉科,541001 [2]桂林医学院附属医院中医科,541001

出　　处：《临床麻醉学杂志》2017年第6期595-597,共3页Journal of Clinical Anesthesiology

基　　金：2012年广西高校学校科研项目(201204LX243);2014年桂林市科学研究与技术开发计划课题(20140310-2-3)

摘　　要：目的探讨电针足三里穴对依托咪酯麻醉大鼠应激反应的影响。方法选择雄性SD大鼠60只,体重280~310g,随机分为六组:空白对照组(C组)、模型组(M组)、依托咪酯60mg/kg组(E1组)、依托咪酯30 mg/kg组(E2组)、依托咪酯60 mg/kg联合电针组(EA1组)和依托咪酯30mg/kg复合电针组(EA2组),每组10只。C组大鼠不进行任何麻醉手术操作,其余五组均建立低位尾干切断模型。建模后,E1组和EA1组大鼠腹腔注射依托咪酯60mg/kg,E2组和EA2组大鼠腹腔注射依托咪酯30mg/kg。EA1组和EA2组大鼠于麻醉状态行双侧足三里穴治疗30min(频率2/100Hz,疏密波,电流强度1mA)。采用ELISA法检测血清ACTH和Cor的浓度,Western blot法检测下丘脑室旁核组织c-fos蛋白的含量。结果 M组ACTH、Cor浓度和c-fos蛋白含量明显高于C组(P<0.05);E1组、E2组、EA1组、EA2组ACTH、Cor浓度和c-fos蛋白含量明显低于M组(P<0.05);E2组、EA1组ACTH、Cor浓度和c-fos蛋白含量明显高于E1组(P<0.05);EA2组ACTH浓度和c-fos蛋白含量明显低于E2组(P<0.05)。结论电针大鼠足三里穴可双向调节依托咪酯对急性应激反应的影响,其机制可能与其调节下丘脑c-fos蛋白水平有关。Objective To explore the effect of electroacupuncture（EA）at tusanli（ST36）on regulation of stress response under different doses of etomidate anesthesia in rats.Methods Sixty male Sprague-Dawley rats,weighing 280-310 g,were randomly divided into control group（group C）,model group（group M）,etomidate 60mg/kg group（group E1）,etomidate 30mg/kg group（group E2）,etomidate 60mg/kg combined with EA group（group EA1）and etomidate 30mg/kg combined with EA group（group EA2）,n=10in each group.All groups received inferior caudal trunk transection at the level between sacral spinal nerve 3and 4（S3,S4）to prepare acute stress response model except group C.Group M received no others treatment.The rats in group E1,group EA1,group E2 and group EA2 were intraperitoneally injected with 60,60,30 and 30mg/kg etomidate,respectively.Group EA1 and group EA2 received EA ST36.The points were stimulated at a frequency of 2/100 Hz with 1mA output and a dilatational wave,which lasted for 30 min.ACTH and Cor levels were measured by ELISA.The c-fos protein expression in hypothalamic tissue was examined by Western blot.Results Compared with group C,ACTH and Cor levels,and hypothalamic expression of c-fos protein in group M were significantly increased（P〈0.05）.Compared with group M,serum ACTH and Cor levels,and hypothalamic expression of c-fos protein in groups E1,E2,EA1 and EA2were significantly decreased（P〈0.05）.Compared with group E1,serum ACTH and Cor levels,and hypothalamic expression of c-fos protein were significantly higher in groups E2 and EA1（P〈0.05）.Compared with group E2,serum ACTH level and hypothalamic expression of c-fos protein were significantly lower in group EA2（P〈0.05）.Conclusion EA at ST36 regulating stress response under etomidate anesthesia in rats is effective and two-way,and the mechanism may be due to the release of neurotransmitters induced c-fos protein in hypothalamus.

关 键 词：电针 依托咪酯 应激 足三里穴 C-FOS蛋白 

分 类 号：R245[医药卫生—针灸推拿学]