不同芽孢杆菌的太赫兹时域光谱特征研究  

Characteristics of different kinds of bacillus by Terahertz time-domain spectroscopy

作　　者：余闻静[1,2] 杨翔[1] 刘羽[1] 赵祥[1] 林钟劝[1] 杨柯[1] 府伟灵[1] 

机构地区：[1]第三军医大学西南医院检验科,重庆400038 [2]中国人民解放军第三二四医院检验科,重庆400023

出　　处：《第三军医大学学报》2017年第13期1315-1320,共6页Journal of Third Military Medical University

基　　金：国家重点基础研究发展计划项目(2015CB755400;2015CB755402);国家自然科学基金重点项目(81430054);军队技术产品研究项目重点项目(BWS13C013)~~

摘　　要：目的应用太赫兹时域光谱技术对常见致病芽孢杆菌进行检测。方法使用太赫兹时域光谱技术对枯草芽孢杆菌、蜡状芽孢杆菌、苏云金芽孢杆菌3种致病菌进行了室温、干燥环境下太赫兹时域光谱测试。得到了样品在0.22.0 THz波段的太赫兹吸收曲线。结果随着频率增加不同含量的芽孢杆菌冻干粉的太赫兹波段的吸收系数是增加的,并且有不同的吸收趋势;此外不同种属的芽孢杆菌的冻干粉和菌落也具有明显不同的吸收系数,这意味着3种样品的分子及官能团的构成和存在状态是不同的,更有力地揭示3种样品的确具有不同的生物成分组成和结构。结论太赫兹时域光谱技术作为一种快速高效、无需标记且无破坏性的探测方法,为芽孢杆菌的检测和鉴别提供了一种行之有效的方法。Objective To detect the common disease-causing bacillus species by Terahertz time- domain spectroscopic （THz-TDS） technique. Methods Three kinds of bacillus species, Bacillus cereus, Bacillus subtilis and Bacillus thuringiensis were tested and investigated with the THz-TDS technique at room temperature in dry environment. The absorption coefficient of 3 bacillus samples were calculated in the frequency of 0.2 - 2.0 THz. Results The absorption coefficients of lyophilized powder containing different amounts of bacillus species were increased as the increase of frequency, and had considerable different absorption trend among the samples. Different coefficients were also observed in the lyophilized powders of different bacillus species and bacterial colonies, which suggested the 3 kinds of species were made up of different molecular vibration and multi-molecular groups in the THz spectrum, and had different biological components and structure in the 3 bacillus samples. Conclusion THz-TDS is a fast, efficient, non-marking and non-destructive detection method, and provides an effective method for the detection and identification of bacillus species.

关 键 词：太赫兹时域光谱 芽孢杆菌 细菌分析 吸收系数 

分 类 号：R312[医药卫生—基础医学] R378