糖代谢相关蛋白1对小鼠糖代谢的调控作用  

The impact of glucose metabolism-related protein 1 on the regulation of glucose metabolism in mouse

作　　者：王宣春[1] 李燕良[1] 冯晓铖[1] 曹心怡 杨志红[1] 胡仁明[1] 闻杰[1] 

机构地区：[1]复旦大学附属华山医院内分泌科,上海200040

出　　处：《中华内分泌代谢杂志》2017年第6期513-518,共6页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金项目（81270904、81370936）;上海市科学技术委员会项目（16140901200,16PJ1401700）

摘　　要：目的探讨糖代谢相关蛋白1（glucose metabolism-related protein 1, GMRP1）对c-Myc基因转录的调控作用，建立基因剔除小鼠模型并研究GMRP1基因缺失对小鼠糖代谢的影响。方法采用染色质免疫共沉淀（ChIP）-PCR筛选GMRP1转录调控的基因，荧光素酶报告基因系统检测GMRP1对c-Myc基因启动子活性的调节。构建GMRP1基因全身剔除小鼠模型，PCR及Western印迹验证小鼠模型是否构建成功。在普通饮食及高脂饮食的喂养条件下，监测基因剔除小鼠及野生小鼠的体重及随机血糖。采用经腹腔注射的葡萄糖耐量试验评估小鼠20周龄及28周龄时的糖代谢水平。结果GMRP1与c-Myc基因的启动子结合，并能激活c-Myc基因的转录。GMRP1基因剔除小鼠与野生C57小鼠比较，无论在普通饮食还是高脂饮食的喂养条件下，体重及随机血糖均无显著差异，糖耐量也无显著性差异（均P〉0.05）。结论GMRP1能激活c-Myc基因的转录，GMRP1基因缺失对小鼠的糖代谢无显著改变。ObjectiveTo investigate the impact of glucose metabolism-related protein 1 （GMRP1） on the regulation of c-Myc gene transcription, to establish GMRP1 gene knockout mouse model, and to study the influence of GMRP1 on glucose metabolism.MethodsChromatin immunoprecipitation-PCR （ChIP-PCR） was utilized to screen out the genes transcriptionally regulated by GMRP1. Luciferase reporter system was applied to assay the regulation of c-Myc promoter activity by GMRP1. GMRP1 knockout mice were constructed and validated by PCR and western blotting. Body weight and random blood glucose were measured in both knockout and wild type mice fed with either chow diet or high-fat diet. The levels of glucose metabolism in mice of 20 or 28 weeks old were evaluated by intraperitoneal glucose tolerance test.ResultsGMRP1 was capable of binding to the promoter of c-Myc gene and activating the expression of c-Myc gene. There were no significant differences in body weight, random blood glucose or glucose tolerance between GMRP1-deficient and wild type mice fed with either chow diet or high-fat diet （all P 〉 0.05）. ConclusionGMRP1 may activate the transcription of c-Myc gene. GMRP1 deficiency exerted no significant effects on mouse glucose metabolism.

关 键 词：糖代谢相关蛋白1 c—Myc 基因剔除 糖代谢 

分 类 号：R587.1[医药卫生—内分泌]