欧前胡素对Aβ_(1-42)致阿尔茨海默病小鼠学习记忆障碍的影响  

Effects of Imperatorin on the Learning and Memory Impairment of Aβ_(1-42)-induced Model of Alzheimer's Disease in Mice

作　　者：余盈[1] 何蔚[1] 

机构地区：[1]赣南医学院药理学教研室江西省脑血管药理重点实验室,赣州341000

出　　处：《天然产物研究与开发》2017年第6期1030-1034,共5页Natural Product Research and Development

摘　　要：研究欧前胡素(imperatorin,Imp)对Aβ_(1-42)致阿尔茨海默病(Alzheimer’s disease,AD)小鼠学习记忆的影响及机制。小鼠脑室内注射Aβ_(1-42)制备AD模型,Imp 2.5 mg/kg和5.0 mg/kg在手术后当天开始腹腔注射给药,1次/d,连续给药13 d,正常对照组和模型组在相同的给药时间点给予溶媒0.1 mL/10 g。第14 d,采用跳台法观察小鼠的学习记忆功能,检测小鼠脑组织内活性氧簇(reactive oxygen species,ROS)、脂质过氧化产物丙二醛(malondialdehyde,MDA)和总抗氧化能力(Total antioxidant capacity,T-AOC)及ATP酶活性。研究显示,同AD模型组比较,Imp 2.5 mg/kg和5.0 mg/kg组小鼠的跳台潜伏期明显延长(P<0.05,P<0.01)和错误次数明显减少(P<0.05,P<0.01),Imp 2.5 mg/kg和5.0 mg/kg组小鼠脑组织中ROS和MDA含量明显降低(P<0.05,P<0.01),Imp 5.0 mg/kg组T-AOC明显增强(P<0.01),Imp 2.5 mg/kg和5.0 mg/kg组Na^+、K^+-ATP与Ca^(2+)-ATP酶的活性明显增强(P<0.05,P<0.01)。研究结果提示,Imp可能通过抑制AD小鼠脑组织中氧化应激损伤,从而改善Aβ_(1-42)致AD小鼠的学习记忆功能。To investigate the effects and mechanisms of imperatorin on learning and memory of beta-amyloid peptide1-42 （Aβ1-42）-induced model of Alzheimer＇s disease （AD） in mice. Mice were injected with Aβ1-42intracerebroventricularly to induce AD model. Imperatorin （2.5 ,Smg/kg） were administered intraperitoneally once daily for 13 days ,the normal control group and the AD model group were given the vehicle （0.1 mL/10 g） according to the same protocol. The learn- ing and memory ability was evaluated by the step-down test, the contents of reactive oxygen species （ROS） and lipid peroxidation products malondialdehyde （MDA） , the total antioxidant capacity （T-AOC） and the activity of Na＋ , K ＋ -ATPase and Ca2 ＋ -ATPase in the brain tissue were assayed. The results showed that as compared to the model group, ad- ministration of imperatorin significantly increased the latency and decreased the number of errors within 5 rain in the step-down test. Furthermore,imperatorin treatment also significantly decreased the level of ROS and MDA, and enhanced T-AOC and the activity of Na＋ , K ＋ -ATPase and Ca2＋ -ATPase in the brain tissue. These results suggested that impera- torin can significantly ameliorate the learning and memory impairment and the mechanism may be partly related to inhib- iting oxidative stress in Aβ1-42-indueed model of dementia in mice.

关 键 词：欧前胡素 AΒ1-42 阿尔茨海默病 学习记忆 跳台法 氧化应激 

分 类 号：R285.5[医药卫生—中药学]