抗人卵泡刺激素受体多克隆抗体的制备及其对实验大鼠骨质疏松的抑制作用  

Inhibition of Osteoporosis in Ovariectomized Rats Using Follicle-stimulating Hormone Receptor Specific Polyclonal Antibody

作　　者：秦瑞坪 李玲霞[1] 马晓玲[1] 席欧彦 赵婷[1] 邱玲玲[1] 李江伟[1] 

机构地区：[1]新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《中国生物工程杂志》2017年第6期9-16,共8页China Biotechnology

基　　金：国家自然科学基金资助项目(81260333)

摘　　要：近年来,卵泡刺激素(follicle-stimulating hormone,FSH)在骨质疏松中的作用得到了证实。卵泡刺激素受体(FSH receptor,FSHR)在成熟的人破骨细胞及破骨细胞前体即单核细胞表面表达,成为阻断FSH作用的潜在靶点。制备了高滴度的兔抗人FSHR多克隆抗体,采用双侧去卵巢手术方法建立SD(Sprague-Dawley)大鼠骨质疏松症动物模型,观察抗FSHR抗体对大鼠实验骨质疏松症的治疗效果。结果显示,卵巢摘除(ovariectomized,OVX)大鼠经抗FSHR多抗和亮丙瑞林(leuprorelin,LE)治疗两周后,与PBS对照组相比,血清FSH和黄体酮(luteinizing hormone,LH)水平显著降低(P<0.05),而雌二醇(estrogen,E2)水平有一定升高,但结果不显著(P>0.05)。另外,骨组织化学染色显示多抗和LE治疗组大鼠骨小梁数目增加,断裂现象较少。这些结果初步表明,采用抗FSHR抗体对SD大鼠骨质疏松症具有一定的治疗作用。There are strong evidences to support the idea that follicle-stimulating hormone （FSH） plays an important role in regulation of bone mass. Owing to its limited expression to gonadal tissues and osteoclasts and its precursor monocytes, FSH receptor may be a good target for FSH blocking therapy. High titers of polyclonal rabbit anti-human FSHR antibody （pAbrsHR） was raised and prepared. The bilateral ovariectomized （OVX） Sprague-Dawley （SD） rats were used as the osteoporosis model for evaluating the effects of pAbvsnR and FSH inhibitor leuprorelin（LE） on the bone loss. The results showed after treatment with pAbrsHR and LE separately, the FSH and luteinizing hormone（LH） levels in OVX groups decreased significantly （P 〈 0. 05 ） compared with PBS treated OVX rats. No significant differences of estrogen （E2） levels were detected between treatment and PBS injected OVX rats （P 〉 0.05 ）. In addition, the number of trabecular bone increased after pAbFSHR treatment in OVX groups in the shin bone histologic analysis. The results suggested targeting against FSHR may work on the treatment of osteoporosis.

关 键 词：骨质疏松症 SD大鼠 抗FSHR多克隆抗体 卵泡刺激素受体 亮丙瑞林 

分 类 号：R392.1[医药卫生—免疫学] Q819[医药卫生—基础医学]