包覆全氟戊烷的介孔氧化硅微球增效高强度聚焦超声表面消融离体牛肝  

Permeating perfluoropentane drops-encapsulated mesoporous silica nanocapsules collaborative high-intensity focused ultrasound surface ablation of bovine liver in vitro

作　　者：丁晓亚 邹建中[1] 王琦[1] 文静[1] 马大钊 

机构地区：[1]重庆医科大学生物医学工程学院省部共建国家重点实验室培育基地-重庆市超声医学工程重点实验室重庆市生物医学工程学重点实验室重庆市微无创医学协同创新中心,重庆400016

出　　处：《中国介入影像与治疗学》2017年第7期439-443,共5页Chinese Journal of Interventional Imaging and Therapy

基　　金：国家自然科学基金(11574039;11274404);国家重点基础研究发展计划(973计划)项目(2011CB707900);国家重大科学仪器设备研制专项(2013YQ03062906);重庆市研究生科研创新项目(CYS14106)

摘　　要：目的探讨包覆全氟戊烷的介孔氧化硅微球(MSNC-PFP)对HIFU表面消融离体牛肝的影响。方法根据MSNC-PFP的浓度将50块离体牛肝平均分为5组:对照组(MSNC-PFP浓度为0)、0.25 mg/ml组、0.50 mg/ml组、1.00mg/ml组、2.00mg/ml组。超声引导沿消融线路径多点注射增效剂(即MSNC-PFP)。通过HIFU线性扫描,以凝固性坏死束组合成面,6个面组合成体,对体腔内区域不直接消融。观察消融中声像图的改变。以TTC染色肉眼观察坏死范围,HE染色光镜下观察坏死程度。测量各组消融体积并评价消融效果,评价指标包括靶区覆盖指数(CI)、靶外体积指数(EI)、能效因子(EEF)。结果当MSNC-PFP浓度在1.00 mg/ml以上时,声像上为团状强回声,3~5 min后消退;MSNC-PFP浓度越高,HIFU辐照区域的灰度值改变越大。0.50、1.00、2.00mg/ml组总消融体积高于对照组,EEF值低于对照组(P均<0.01)。1.00、2.00mg/ml组的CI及EI值均高于其余各组(P均<0.05)。肉眼观察凝固性坏死区表现为灰白色,未坏死区则表现为红色。光镜下见当MSNC-PFP浓度≥0.50mg/ml时,形成的凝固性坏死带完整。结论采用MSNC-PFP可增大HIFU表面消融离体牛肝的消融体积,减低EEF,从而提高辐照效率。Objective To discusse the influence of permeating perfluoropentane drops-encapsulated mesoporous silica nanocapsules （MSNC-PFP） on HIFU surface ablation in vitro bovine liver. Methods Fifity bovine-liver tissues were divided into 5 groups： Control group, 0.25 mg/ml group, 0.50 mg/ml group, 1.00 mg/ml group, 2.00 mg/ml group according to the concentration of MSNC-PFP. The synergistic agent was injected along the ablation line path under the guiding of ultrasound. The surface ablation model was structured by 6 coagulation necrosis side by using HIFU linear scanning, inner region without melting, and the ultrasonographic changes were observed in the melt area. The coagulation necrosis range was assessed by TTC staining, and necrosis degree was observed by HE staining. The coagulation necrosis volume was measured, the coverage index （CI）, external volume index （EI）, energy efficiency factor （EEF） were used to evaluate the ablation effect in each group. Results The MSNC-PFP more than 1.00 mg/ml was shown as strong echo in ultrasonic image, fade after 3-5 min. The grey value was changed in HIFU ablation area related to the concentration of MSNC-PFP. The total ablation volume of 0.50 mg/ml group, 1.00 mg/ml group, 2.00 mg/ml group were more than control group, the EEF was lower than that of the control group （all P〈0.01）. The CI and EI of 1.00 mg/ml group and 2.00 mg/ml group were higher than those of the other group （all P〈0.01）. The coagulation necrosis became complete when the concentration of MSNC-PFP above 0.50 mg/ml. Conclusion MSNC-PFP can increase the volume of coagulation necrosis, decrease the EEF and increase the efficiency of HIFU surface ablation of bovine liver in vitro.

关 键 词：全氟戊烷的介孔氧化硅微球 高强度聚焦超声消融术 离体 牛 肝 

分 类 号：R730.5[医药卫生—肿瘤]