胡杨PeAPY1和PeAPY2调控拟南芥耐盐机制研究  

PeAPY1 and PeAPY2 of Populus euphratica regulating salt tolerance in Arabidopsis thaliana

作　　者：邓佳音[1] 张艳丽[1] 张一南[1] 赵瑞[1] 李金克[2] 周晓阳[2] 刘香芬 陈少良[1] 

机构地区：[1]北京林业大学生物科学与技术学院 [2]北京林业大学分析测试中心 [3]河北石家庄市城市水系管理处

出　　处：《北京林业大学学报》2017年第6期13-21,共9页Journal of Beijing Forestry University

基　　金：国家自然科学基金项目(31270654;31570587);教育部科学技术研究(科学技术类)项目(113013A);教育部创新团队发展计划项目(IRT13047);北京市自然科学基金项目(6172024);高等学校学科创新引智计划项目(111project;B130007)

摘　　要：本文研究了胡杨apyrase基因(Pe APY1和Pe APY2)对植物耐盐性的影响。以Pe APY1和Pe APY2过表达拟南芥、拟南芥Atapy1和Atapy2突变体、野生型(WT)拟南芥及空载体(VC)为实验材料,研究盐胁迫条件下的植物根长、相对电导率、细胞活力、H_2O_2水平、eATP浓度、抗氧化酶活性的变化。研究结果显示:低盐浓度(50 mmol/L NaCl)对拟南芥各基因型的生长和生理生化指标没有显著影响;而高盐浓度(100 mmol/L NaCl)抑制了各株系的根长生长、细胞活力和抗氧化酶(超氧化物歧化酶/SOD、抗坏血酸过氧化酶/APX、过氧化氢酶/CAT)活性,却提高了相对电导率、H_2O_2水平和eATP浓度。但与Atapy突变体株系相比较,Pe APY过表达株系受高盐胁迫的影响较小。主要是由于Pe APY1/2的过表达提高了apyrase酶活,下调了eATP浓度及其诱发的活性氧水平,同时,过表达株系还通过保持抗氧化酶活性来抑制H_2O_2的水平,从而降低活性氧及其对膜脂的过氧化,保持了膜的稳定性、细胞活力和生长,最终提高了植物耐盐性。We investigated the effects of Populus euphratica apyrase genes（ PeA PY1 and PeA PY2） on salt tolerance of higher plants. Using PeA PY1 and PeA PY2 transgenic plants,Atapy1 and Atapy2 mutants,Arabidopsis wild type（ WT） and vector controls（ VC）,we examined the effects of Na Cl on root length,relative electrolyte leakage,cell viability,H2O2,extracellular ATP（ eATP） and antioxidant enzyme activities. Results showed that the low salinity（ 50 mmol/L Na Cl） had no significant effects on root growth,physiological and biochemical parameters. However, a high salinity（ 100 mmol/L Na Cl）markedly reduced root length,cell viability and activities of antioxidant enzymes（ superoxide dismutase/SOD,ascorbate peroxidase/APX,catalase/CAT）. In contrast,the high Na Cl increased relative electrolyte leakage,H2O2,and eATP levels in all the tested lines. PeA PY1/2-transgenic lines exhibited a less pronounced effects as compared with the Arabidopsis Atapy mutants under high salinity. We inferred that the overexpression of PeA PY1/2 led to an up-regulated apyrase,which hydrolyzed eATP and downregulated theATP induction of H2O2. Moreover,PeA PY1/2-transgenic lines maintained activity of antioxidant enzymes under salt stress. This would reduce the membrane peroxidation induced by thereactive oxygen species. As a result,the membrane integrity and cell viability were less affected by salt stress,leading to an increased salt tolerance in PeA PY1/2-transgenic plants.

关 键 词：PeAPY1 PeAPY2 盐胁迫 膜透性 胞外ATP H2O2 

分 类 号：S792.11[农业科学—林木遗传育种] Q945[农业科学—林学]