Pronuclear formation by ICSI using chemically activated ovine oocytes and zona pellucida bound sperm  

作　　者：J.E.Hernández-Pichardo Y.Ducolomb S.Romo M.E.Kjelland R.Fierro F.Casillas M.Betancourt 

机构地区：[1]División de Ciencias Biológicas y de la Salud,Departamento de Producción Agrícola y Animal,Universidad Autónoma Metropolitana-Xochimilco,Ciudad de Mexico,Mexico [2]Division de Ciencias Biologicas y de la Salud,Departamento de Ciencias de la Salud,,Universidad Autónoma Metropolitana-Iztapalapa,San Rafael Atlixco 186,CP 09340 Ciudad de Mexico,Mexico [3]Departamento de Ciencias Pecuarias,Facultad de EstudiosSuperiores Cuautitlan,UNAM,Ciudad de Mexico,Estado de Mexico,Mexico [4]Conservation,Genetics&Biotech,LLC,Valley City,ND,USA [5]Division de Ciencias Biologicas y de la Salud,Departamento de Biologia de la Reproduccion,Universidad Autonoma Metropolitana-lztapalapa,Ciudad de Mexico,Mexico [6]Doctorado en Biologia Experimental,Universidad Autonoma Metropolitana-lztapalapa,Ciudad de Mexico,Mexico

出　　处：《Journal of Animal Science and Biotechnology》2017年第2期370-377,共8页畜牧与生物技术杂志（英文版）

摘　　要：Background： In order to improve ICSI, appropiate sperm selection and oocyte activation is necessary. The objective of the present study was to determine the efficiency of fertilization using ICSI with chemically activated ovine oocytes and sperm selected by swim up（SU） or swim up ＋ zona pellucida（SU ＋ ZP） binding.Results： Experiment 1, 4–20 replicates with total 821 in vitro matured oocytes were chemically activated with ethanol, calcium ionophore or ionomycin, to determine oocyte activation（precense of one PN）. Treatments showed similar results（54, 47, 42 %, respectively） but statistically differents（P 0.05）.Conclusions： Chemical activation induces higher ovine oocyte activation than mechanical activation. Ethanol slightly displays higher oocyte activation than calcium ionophore and ionomicine. Sperm selection with SU ＋ ZP increased AR/A and AR/D rates in comparison with SU in fresh and frozen-thawed sperm. According to this, in terms of fertilization rates, chemical activation after ICSI increased oocyte PN formation compared to mechanical activation. Also, fresh sperm treated with SU and SU ＋ ZP were significantly different than frozen-thawed sperm,but between sperm treatments no significant differences were obtained.Background： In order to improve ICSI, appropiate sperm selection and oocyte activation is necessary. The objective of the present study was to determine the efficiency of fertilization using ICSI with chemically activated ovine oocytes and sperm selected by swim up（SU） or swim up ＋ zona pellucida（SU ＋ ZP） binding.Results： Experiment 1, 4–20 replicates with total 821 in vitro matured oocytes were chemically activated with ethanol, calcium ionophore or ionomycin, to determine oocyte activation（precense of one PN）. Treatments showed similar results（54, 47, 42 %, respectively） but statistically differents（P 0.05）.Conclusions： Chemical activation induces higher ovine oocyte activation than mechanical activation. Ethanol slightly displays higher oocyte activation than calcium ionophore and ionomicine. Sperm selection with SU ＋ ZP increased AR/A and AR/D rates in comparison with SU in fresh and frozen-thawed sperm. According to this, in terms of fertilization rates, chemical activation after ICSI increased oocyte PN formation compared to mechanical activation. Also, fresh sperm treated with SU and SU ＋ ZP were significantly different than frozen-thawed sperm,but between sperm treatments no significant differences were obtained.

关 键 词：Ethanol ICSI Oocyte activation Ovine Pronucleus Zona pellucida 

分 类 号：S826[农业科学—畜牧学]