外源性硫化氢对内毒素致急性肺损伤小鼠肺巨噬细胞表型转化的影响  

Effect of exogenous hydrogen sulfide on phenotypic transformation of alveolar macrophages in a mouse model of endotoxin-induced acute lung injury

作　　者：秦文瀚[1] 杨从文[1] 杨贞[1] 鲁开智[1] 甯交琳[1] 

机构地区：[1]第三军医大学西南医院手术麻醉科,重庆市400038

出　　处：《中华麻醉学杂志》2017年第5期609-612,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81470267）

摘　　要：目的 评价外源性硫化氢对内毒素致急性肺损伤小鼠肺巨噬细胞表型转化的影响。方法 清洁级健康雄性C57BL/6小鼠30只，8周龄，体重18～20 g，采用随机数字表法分为3组（n＝10）：假手术组（Sham组）、急性肺损伤组（ALI组）和外源性硫化氢组（NaHS组）。Sham组气管滴注和腹腔注射生理盐水；ALI组和NaHS组气管滴注脂多糖20mg/kg，每天分别腹腔注射生理盐水和硫氢化钠（28 μmol/kg）各100 μl。给予脂多糖后3 d时测定肺系数，免疫组化法检测肺组织诱导型一氧化氮合酶（iNOS）和精氨酸酶的表达；观察肺组织病理学结果并进行半定量肺损伤评分（IQA）。结果 与Sham组比较，ALI组肺系数和IQA升高，肺组织iNOS和精氨酸酶表达上调（P〈0.05）；与ALI组比较，NaHS组肺系数和IQA降低，肺组织iNOS表达下调（P〈0.05），精氨酸酶表达差异无统计学意义（P〉0.05）。结论 外源性硫化氢通过抑制肺巨噬细胞表型转化为M1型减轻小鼠内毒素性急性肺损伤。Objective To evaluate the effect of exogenous hydrogen sulfide on phenotypic transformation of alveolar macrophages in a mouse model of endotoxin-induced acute lung injury （ALI）.Methods Thirty pathogen-free healthy male C57BL/6 mice, aged 8 weeks, weighing 18-20 g, were divided into 3 groups （n=10 each） using a random number table： sham operation group （group Sham）, group ALI and exogenous hydrogen sulfide group （group NaHS）. In group Sham, normal saline was intratracheally instilled and intraperitoneally injected.In ALI and NaHS groups, lipopolysaccharide 20 mg/kg was intratracheally instilled, and normal saline and sodium hydrosulfide （28 μmol/kg） 100 μl were intraperitoneally injected, respectively, every day.Mice were sacrificed at day 3 after administration of lipopolysaccharide, and lungs were removed for measurement of the lung coefficient and expression of inducible nitric oxide synthase （iNOS） and arginase （by immunohistochemistry） and for microscopic examination of the pathological changes.Lung injury was evaluated by the index of quantitative assessment （IQA）.Results Compared with group Sham, the lung coefficient and IQA were significantly increased, and the expression of iNOS and arginase in lung tissues was up-regulated in group ALI （P〈0.05）. Compared with group ALI, the lung coefficient and IQA were significantly decreased, the expression of iNOS in lung tissues was down-regulated （P〈0.05）, and no significant change was found in the expression of arginase in lung tissues in group NaHS （P〉0.05）.Conclusion Exogenous hydrogen sulfide mitigates endotoxin-induced ALI through inhibiting phenotypic transformation of alveolar macrophages to M1 subtype in mice.

关 键 词：硫化氢 呼吸窘迫综合征 成人 内毒素类 巨噬细胞 表型 

分 类 号：R563.8[医药卫生—呼吸系统]