脓毒症小鼠脾CD27和CD11b标记NK细胞亚群的变化  

Changes in natural killer cell subsets defined by CDllb and CD27 in spleen of septic mice

作　　者：乌兰 王国林 李财峰[2] 张瑜 郭素倩 王志强[2] 彭民[2] 崔海龙[2] 

机构地区：[1]天津麻醉学研究所,300052 [2]天津医科大学总医院重症医学科

出　　处：《中华麻醉学杂志》2017年第5期618-620,共3页Chinese Journal of Anesthesiology

基　　金：天津医科大学总医院青年孵育基金（ZYYFY2014003）

摘　　要：目的 评价脓毒症小鼠脾CD27和CD11b标记自然杀伤细胞（NK细胞）亚群的变化。方法 清洁级健康雄性C57BL/6小鼠168只，体重20～30 g，8～10周龄，采取随机数字表法分为2组（n＝84）：假手术组（Sham组）和脓毒症组（Sep组）。采用盲肠结扎穿孔法制备小鼠脓毒症模型。每组取小鼠30只，观察术后4 d内存活情况，计算生存率。于术后2、4、6、24、48和72 h时（T1～6）随机取6只小鼠，摘除眼球取血，采用ELISA法检测血清TNF-α和IL-10的浓度；于T4～6时随机取6只小鼠，断颈处死取脾脏，采用流式细胞仪检测NK细胞及其亚群的百分比。结果 与Sham组比较，Sep组不同时点小鼠生存率降低，T1～3，6时血清TNF-α浓度和T3～6时血清IL-10浓度升高，T4～6时脾NK细胞百分比和T5时CD27－CD11b＋NK细胞百分比降低，CD27＋CD11b＋和CD27＋CD11b－NK细胞百分比升高，T4，5时CD27－CD11b－NK细胞百分比降低，T6时CD27－CD11b－NK细胞百分比升高（P〈0.05）。结论 脾CD27和CD11b标记NK细胞亚群变化在小鼠脓毒症发生发展中起重要作用。Objective To evaluate the changes in natural killer（NK）cell subsets defined by CD11b and CD27 in the spleen of septic mice.Methods A total of 168 pathogen-free healthy male C57BL/6 mice, weighing 20-30 g, aged 8-10 weeks, were divided into 2 groups（n=84 each）using a random number table： sham operation group（Sham group）and sepsis group（Sep group）. Sepsis was induced by cecal ligation and puncture in chloral hydrate-anesthetized mice.Thirty mice in each group were selected to assess the survival within 4 days after operation, and the survival rate was calculated.At 2, 4, 6, 24, 48 and 72 h after operation（T1-6）, 6 mice were randomly selected, and blood samples were taken from the eyeballs for determination of serum tumor necrosis factor-alpha and interleukin-10 concentrations by enzyme-linked immunosorbent assay.Six mice were randomly selected at T4-6 and sacrificed, and the spleens were removed for measurement of the percentage of NK cells and their subsets by flow cytometry.Results Compared with Sham group, the survival rate was significantly decreased at different time points, the serum necrosis factor-alpha concentration at T1-3, 6 and serum interleukin-10 concentration at T3-6 were increased, the percentage of NK cells was decreased at T4-6, the percentage of CD27-CD11b＋ NK cells was decreased and the percentage of CD27＋ CD11b＋ and CD27＋ CD11b-NK cells was increased at T5, the percentage of CD27-CD11b-NK cells was decreased at T4, 5, and the percentage of CD27-CD11b-NK cells was increased at T6 in Sep group（P〈0.05）.Conclusion Changes in NK cell subsets defined by CD11b and CD27 in the spleen play an important role in the development of sepsis in mice.

关 键 词：脓毒症 杀伤细胞 天然 抗原 CD27 抗原 CD11b 

分 类 号：R459.7[医药卫生—急诊医学]