乳化异氟醚后处理对大鼠心肌缺血再灌注时线粒体自噬的影响  

Effect of emulsified isoflurane postconditioning on mitophagy during myocardial ischemia-reperfusion in rats

作　　者：付红光[1] 杨现会[1] 吕淼淼[1] 李彬[1] 李文波[1] 董铁立[1] 

机构地区：[1]郑州大学第二附属医院麻醉科,450014

出　　处：《中华麻醉学杂志》2017年第5期625-628,共4页Chinese Journal of Anesthesiology

摘　　要：目的 评价乳化异氟醚后处理对大鼠心肌缺血再灌注时线粒体自噬的影响。方法 清洁级健康雄性SD大鼠48只，4～5月龄，体重250～300 g，采用随机数字表法分为4组（n＝12）：假手术组（S组）、缺血再灌注组（I/R组）、脂肪乳组（F组）和乳化异氟醚后处理组（EIP组）。采用缺血30 min再灌注120 min的方法制备大鼠心肌缺血再灌注损伤模型，于再灌注前3 min时EIP组持续静脉输注8%乳化异氟醚（2 ml/kg）8 min，F组持续静脉输注30%脂肪乳8 min。采用TTC法确定心肌梗死体积比，TUNEL法检测凋亡心肌细胞，计算细胞凋亡指数，测定线粒体膜电位，Western blot法检测心肌细胞微管相关蛋白1轻链3（LC3）、Beclin1、P62、PINK1和Parkin的表达。结果 与S组比较，I/R组、F组和EIP组心肌梗死体积比和细胞凋亡指数增加，线粒体膜电位降低，LC3、Beclin1、PINK1和Parkin表达上调，P62表达下调（P〈0.05）；与I/R组比较，EIP组心肌梗死体积比和细胞凋亡指数降低，线粒体膜电位升高，LC3、Beclin1、PINK1和Parkin表达下调，P62表达上调（P〈0.05）；与F组比较，EIP组心肌梗死体积比和细胞凋亡指数降低，线粒体膜电位升高，LC3、Beclin1、PINK1、Parkin表达下调，P62表达上调（P〈0.05）。结论 乳化异氟醚后处理减轻大鼠心肌缺血再灌注损伤的机制与抑制线粒体自噬有关。Objective To evaluate the effect of emulsified isoflurane postconditioning on mitophagy during myocardial ischemia-reperfusion（I/R）in rats.Methods Forty-eight pathogen-free healthy male Sprague-Dawley rats, aged 4-5 months, weighing 250-300 g, were divided into 4 groups（n=12 each）using a random number table： sham operation group（group S）, group I/R, fat emulsion group（group F）and emulsified isoflurane postconditioning group（group EIP）. Myocardial I/R was induced by occlusion of the anterior descending branch of the left coronary artery for 30 min followed by 120 min of reperfusion in pentobarbital sodium-anesthetized rats.Starting from 3 min before reperfusion, 8% emulsified isoflurane 2 ml/kg was intravenously infused over 8 min in group EIP, while 30% fat emulsion 2 ml/kg was intravenously infused over 8 min in group F. Rats were sacrificed at the end of reperfusion, and hearts were removed for measurement of the myocardial infarct size（by 2, 3, 5-triphenyltetrazolium chloride staining）, cell apoptosis（by TUNEL）, mitochondrial membrane potential and expression of microtubule-associated protein 1 light chain 3（LC3）, Beclin1, P62, PINK1 and Parkin in cardiomyocytes（by using Western blot）. Apoptosis index（AI）was calculated.Results Compared with group S, the myocardial infarct size and AI were significantly increased, the mitochondrial membrane potential was decreased, the expression of LC3, Beclin1, PINK1 and Parkin was up-regulated, and the expression of P62 was down-regulated in I/R, F and EIP groups（P〈0.05）. Compared with group I/R, the myocardial infarct size and AI were significantly decreased, the mitochondrial membrane potential was increased, the expression of LC3, Beclin1, PINK1 and Parkin was down-regulated, and the expression of P62 was up-regulated in group EIP（P〈0.05）. Compared with group F, the myocardial infarct size and AI were significantly decreased, the mitochondrial membrane potential was increased, the expression of LC3, Beclin1, PINK1 and

关 键 词：异氟醚 脂肪乳剂 静脉注射用 心肌再灌注损伤 线粒体 自噬 缺血后处理 

分 类 号：R614[医药卫生—麻醉学]