MicroRNA-155通过CEH影响巨噬细胞泡沫化过程  

MicroRNA-155 affects macrophage foaming through CEH

作　　者：张凤香 李晨光 孙大鹏 

机构地区：[1]锦州医科大学附属第一医院心外科,辽宁省锦州市121001

出　　处：《中国动脉硬化杂志》2017年第6期553-558,共6页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金(81541099);辽宁省自然科学基金(2016010330-301)

摘　　要：目的探讨microRNA-155(miR-155)通过干扰人THP-1单核巨噬细胞内胆固醇酯水解酶(CEH)的表达来影响巨噬细胞泡沫化形成的机制。方法通过油红O染色和和高效液相色谱法(HPLC)分析胆固醇酯(CE)含量,观察阳性细胞率和细胞内胆固醇酯(CE)含量的变化情况。Western blot检测上述各组细胞内CEH、三磷酸腺苷结合盒转运体A1(ABCA1)、清道夫受体(SR-A)的表达变化。结果转染miR-155 mimics的细胞阳性比率、胆固醇酯、游离胆固醇(FC)、总胆固醇(TC)含量均随着时间的延长明显下降(P<0.05);miR-155 mimics组细胞阳性比率、胆固醇含量和SR-A的表达均被明显降低(P<0.05),而CEH和ABCA1的表达被明显增强(P<0.05);miR-155 mimics+siRNA-CEH和siRNA-CEH组细胞阳性比率、胆固醇含量和SR-A的表达均明显增加(P<0.05),而CEH和ABCA1的表达被明显降低(P<0.05)。结论高表达的miR-155可以明显抑制巨噬细胞泡沫化的形成,该作用可能是通过增强巨噬细胞内CEH的表达进而影响ABCA1和SR-A等相关基因的表达来得以实现。Aim To study the mechanism of microRNA-155（miR-155） depending on cholesteryl ester hydrolase （CEH） to influence foam cells formation.Methods Oil red O staining and HPLC were used to analyze cholesteryl ester content,and the positive cells and cholesterol ester content were observed.Western-blot was used to the expression of CEH,ATP binding cassette transporter A1（ABCA1）,scavenger receptor AⅠ（SR-A）.Results The positive cell ratio and the cholesterol efflux were dramatically decreased by miR-155 mimics（P〈0.05）;the positive cell ratio,the cholesterol efflux,the expression of SR-A were significantly decreased （P〈 0.05）,while the expression of CEH and ABCA1 were significantly increased in miR-155 mimics group respectively（P〈0.05）.The expression of SR-A,the positive cell ratio,the cholesterol efflux were significantly increased（P〈0.05）,while the expression of CEH and ABCA1 were significantly decreased in microRNA-155＋siRNA-CEH group and siRNA-CEH group respectively（P〈0.05）.Conclusion The high expression of microRNA-155 can inhibit the formation of foam macrophages,which may be enhanced by CEH affecting the expression of ABCA1 and SR-A.

关 键 词：MicroRNA-155 胆固醇酯水解酶 巨噬细胞 

分 类 号：R446.1[医药卫生—诊断学]