光合细菌分离鉴定及其对草鱼鱼苗养殖水质的影响  

Isolation and Identification of Photosynthesis Bacteria and Its Effect on the Water Quality in Aquaculture Ponds of Grass Carp Fry

作　　者：宋东蓥[1] 张石云[1] 张建新[1] 李学军[1] 刘莹丽[1] 连跃斌 

机构地区：[1]河南师范大学水产学院,河南新乡453007

出　　处：《河南师范大学学报（自然科学版）》2017年第5期121-124,共4页Journal of Henan Normal University(Natural Science Edition)

基　　金：河南省高等学校重点科研项目(16B240002)

摘　　要：从养殖池塘底泥样品中分离纯化得到紫色非硫光合细菌1株,通过观察分离菌株的细胞形态结构和16SrDNA序列分析,鉴定该株光合细菌属于沼泽红假单胞菌(Rhodops eudomonaspalusteris).采用三级扩大培养法生产光合细菌,活菌数达3×10~9 cfu/mL.在草鱼鱼苗养殖池塘投加光合细菌,可维持池塘透明度,明显降低亚硝酸盐和氨氮含量.A strain of purple non-sulfur photosynthetic bacteria was isolated from the mud samples of aquaculture ponds.According to the cell morphology and the sequence analysis of 16 SrDNA,the strain was identified as Rhodopseudomonaspalusteris.The number of living bacterium were 3x10^9 cfu/mL through a three-stage progressive scale-up culture.After adding the bacteria to aquaculture ponds of grass carp fries,the transparency of the experiment ponds can be kept,and the contents of ammonia andnitrite nitrogenobviously decreased.

关 键 词：光合细菌 草鱼鱼苗 氨氮 亚硝酸盐 

分 类 号：Q178[生物学—水生生物学] Q945[生物学—普通生物学]