克唑替尼对人卵巢癌细胞系SKOV3细胞增殖的影响  

Effects of Crizotinib on Proliferation of Human Ovarian Cancer Cell Line SKOV3

作　　者：邓梅君 刘俊[2] 李贵玲[2] 

机构地区：[1]南方医科大学第三附属医院肿瘤科,广州510630 [2]华中科技大学同济医学院附属协和医院肿瘤中心,武汉430022

出　　处：《医药导报》2017年第7期727-730,共4页Herald of Medicine

基　　金：国家自然科学基金资助项目(81472708)

摘　　要：目的探索人卵巢浆液性腺癌细胞系SKOV3中间变性淋巴瘤激酶(ALK)的表达及其对克唑替尼的反应。方法采用蛋白免疫印迹法(Western blotting)及免疫细胞化学法检测ALK蛋白在人卵巢癌细胞系SKOV3中的表达;细胞增殖实验检测ALK抑制药克唑替尼对SKOV3细胞增殖的影响。结果 Western blotting结果显示,SKOV3细胞中有ALK蛋白表达;免疫细胞化学结果显示,ALK在SKOV3细胞质中有表达。细胞增殖实验显示,克唑替尼≤10~6nmol·L^(-1)时SKOV3细胞活力与对照组比值均接近1,达到10~7nmol·L^(-1)时SKOV3细胞活力值接近64%。结论人卵巢癌细胞系SKOV3细胞中有ALK蛋白表达,但其对克唑替尼治疗不敏感。Objective To explore the expression of anaplastic lymphoma kinase （ ALK） in human ovarian serous adenocarcinoma cell line SKOV3 and to further investigate the effect of crizotinib on SKOV3 cells. Methods The expression of ALK in SKOV3 cells were examined by Western blotting and immunocytochemical methods,and the effect of ALK inhibitor crizotinib on proliferation of SKOV3 cells were evaluated by cell proliferation assay. Results Both Western blotting and immunocytochemical methods show-ed the expression of ALK in SKOV3 cells.The results of cell proliferation assay suggested that the cell viability of SKOV3 cells was close to 1 when the drug concentration was less than 10^6 nmol · L^-1 ,and close to 64% when the drug concentration was up to 10^7nmol· L^-1 Conclusion ALK is overexpressed in human ovarian serous adenocarcinoma cell line SKOV3,but SKOV3 cells were insensitive to the therapy of ALK inhibitor crizotinib.

关 键 词：克唑替尼 癌 卵巢 SKOV3细胞 间变性淋巴瘤激酶 

分 类 号：R979.1[医药卫生—药品] R737.31[医药卫生—药学]