超高效液相色谱法测定人血浆水杨酸浓度不确定度评定  

Uncertainty Evaluation for the Determination of Salicylic Acid in Human Plasma by UPLC

作　　者：赵坤[1] 梅升辉[1] 李新刚[1] 赵志刚[1] 

机构地区：[1]首都医科大学附属北京天坛医院药学部,北京100050

出　　处：《医药导报》2017年第7期737-740,共4页Herald of Medicine

基　　金：国家自然科学基金资助项目(81503157);北京市自然科学基金资助项目(7164256)

摘　　要：目的评定超高效液相色谱(UPLC)法测定人血浆水杨酸浓度的不确定度。方法分析UPLC法测定人血浆水杨酸浓度过程中的不确定度来源,计算并进行合成和扩展。结果水杨酸在最低定量下限浓度(0.23μg·mL^(-1))和高质控浓度(39.43μg·mL^(-1))的扩展不确定度分别为0.014和7.34μg·mL^(-1)(P=95%,k=2)。结论UPLC法测定人血浆水杨酸浓度不确定度,在最低定量下限浓度和高质控浓度时主要由回收率、生物样品配制和重复性引入。Objective To evaluate the uncertainty for the determination of salicylic acid in human plasma by ultra perfor^nance liquid chromatography （ UPLC）. Methods All sources of uncertainty in the whole process of salicylic acid determination were analyzed,then the combined and expanded uncertainty were evaluated. Results The expanded uncertainty at concentrations of the lowest limit of quantitation（0.23μg·mL^-1 ） and a high level（39.43 μg·mL^-1 ） of salicylic acid（ P = 95%,A=2） was 0.014 and 7.34 μg·mL^-1 , respectively. Conclusion The uncertainty of salicylic acid determination in human plasma by UPLC was mainly caused by recovery, repeatability and sample preparation at the lowest limit of quantitation and high qulity control concentration.

关 键 词：水杨酸 不确定度评定 色谱法 超高效液相 血浆 人 

分 类 号：R972.1[医药卫生—药品]