霉茶蛋白对原发性高血压大鼠心脏的影响  

Effect of Meicha Protein on the Heart of Spontaneously Hypertensive Rats

作　　者：董萌[1] 郑国华[1] 胡俊杰[1] 张宝徽[1] 吴勇[1] 

机构地区：[1]湖北中医药大学中药资源与中药复方教育部重点实验室老年病中药新产品湖北省协同创新中心,武汉430065

出　　处：《医药导报》2017年第7期746-750,共5页Herald of Medicine

基　　金：国家自然科学基金资助项目(81173502)

摘　　要：目的观察霉茶蛋白对原发性高血压大鼠(SHR)心脏的保护作用,并探讨其作用机制。方法将40只SHR随机分为模型对照组(纯化水)、霉茶蛋白小剂量组(70 mg·kg^(-1))、霉茶蛋白大剂量组(140 mg·kg^(-1))和复方罗布麻片组(50 mg·kg^(-1)),每组10只。大鼠每天灌胃2次,连续7周,隔周测量血压。末次给药后1 h,颈总动脉取血,剥离心脏组织,计算心脏指数;取部分组织行病理组织学检查;检测血清一氧化氮(NO)、内皮素(ET-1)含量以及组织血管紧张素转化酶(ACE)和血管紧张素Ⅱ(AngⅡ)的m RNA表达水平。结果霉茶蛋白可明显降低SHR血压,对大鼠的心脏指数影响不明显,对大鼠心脏组织具有一定保护作用。与模型对照组比较,霉茶蛋白大、小剂量组NO含量明显增加,ET-1含量降低(P<0.01);与模型对照组比较,霉茶蛋白大剂量组心脏ACE、AngⅡ、CYP11B2 m RNA表达量明显降低(P<0.01)。结论霉茶蛋白可降低SHR血压,改善心脏组织病理改变,其作用机制可能与增加血清NO含量、降低血清ET-1含量、下调心脏组织ACE和AngⅡm RNA表达有关。Objective To observe the protective effect of meicha protein on the heart of spontaneously hypertensive rats（SHR）,and explore its mechanism. Methods Fourty healthy SHR rats were randomly divided into 4 groups：model control group,Meicha protein low dose group（70 mg·kg^-1）、Meicha protein high dose group（140 mg·kg^-1）,Compound Kendir Leaves Tablets group（50 mg·kg^-1）,n=10.The rats were orally administered twice daily by gavage for seven weeks,measuring blood pressure in each group fort nightly.1 h after the last administration,drawing off the blood from carotid,stripping off the heart tissue,and the organ index was calculated; Taking a part of the tissue with 4% paraformaldehyde for Pathological histology.Detection of serum NO,ET-1 levels as well as the organization of the ACE and Ang II mRNA expression to explore the mechanism of its buck. Results Meicha protein could significantly reduce the blood pressure of SHR; The impact on the rat organ coefficient was not obvious,but had a protective effect on heart tissue.Compared with the model control group,the contents of NO an ET-1 were significantly increased（P〈0.01）.Compared with the model group,the high dose of Meicha protein could induce ACE,AngⅡ,CYP11B2.The expression of mRNA was significantly decreased（P〈0.01）. Conclusion The possible mechanism of Meicha protein antihypertensionis relevant to increase the content of NO in serum,reduce the content of ET-1 in serum,reduce mRNA expression of ACE and AngⅡin cardiac tissue.

关 键 词：霉茶蛋白 高血压 原发性 一氧化氮 内皮素 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]