红花龙胆血清药物化学初步研究  

Preliminary Study on Serum Pharmacochemistry of Gentiana rhodantha

作　　者：赵琳珺 罗君[3] 卿娟[4] 武春兰[3] 张丽艳[1] 叶世芸[1] 

机构地区：[1]贵阳中医学院,贵阳550002 [2]贵阳新天社区卫生服务中心,贵阳550008 [3]贵阳中医学院第一附属医院药剂科,贵阳550001 [4]贵州省修文县疾病预防控制中心,贵阳550200

出　　处：《医药导报》2017年第7期750-752,共3页Herald of Medicine

基　　金：贵州省科技厅贵阳中医学院联合基金(黔科合中药字[2012]LKZ7059);贵州省中医药管理局中医药;民族医药科学技术研究课题(QZYY2012-04;QZYY2016-099);贵州省科技厅贵阳中医学院第一附属医院联合基金资助项目(黔科合LH字[2015]7801);贵阳中医学院院内项目(贵中医科院内[2016]55号);苗族医学研究协同创新中心项目(黔教合协同创新字[2015]05)

摘　　要：目的研究红花龙胆血清药物化学,探讨红花龙胆药效作用的物质基础。方法采用血清药物化学研究方法,在建立红花龙胆高效液相色谱(HPLC)指纹图谱的基础上,比较红花龙胆提取物、红花龙胆含药血清、空白血清HPLC指纹图谱差异,确认红花龙胆提取液灌胃后大鼠含药血清的移行成分。结果在红花龙胆含药血清中发现20个入血成分,4个为原型成分,其余16个可能为原型成分代谢产物。结论 20个入血成分可能是红花龙胆的体内直接作用物质,对其进行深入研究可能有助于阐明红花龙胆的药效物质基础及药效机制。Objective To explore the bioactive constituents of Gentiana rhodantha for its serum pharmacochemistry research. Methods The blood migrating constituents of Gentiana rhodantha were determined by comparing the HPLC fingerprints of the aqueous extracts,drug contained serum and blank serum. Results Twenty compounds were detected in drug contained serum,four among which were original constituents of Gentiana rhodantha, the rest might be metabolites of the original ingredients. Conclusion These twenty constituents absorbed in blood could be the bioactive components of Gentiana rhodantha .Further studies will be useful to clarify the bioactive constituents and action mechanisms of Gentiana rhodantha

关 键 词：红花龙胆 血清药物化学 指纹图谱 色谱法 高效液相 

分 类 号：R286[医药卫生—中药学] R969[医药卫生—中医学]