雷公藤多苷在小鼠胰岛移植中的抗排斥作用  

Anti-rejection Effect of Tripterygium Glycosides on Allogeneic Islet Transplantation in Mice

作　　者：高歌[1] 傅红兴[1] 徐福远 邱凯燕 蒋煊[1] 陈佳妮[1] 陈心舒 

机构地区：[1]温州医科大学药学院,温州325035 [2]浙江省乐清市石帆街道社区卫生服务中心,乐清325608

出　　处：《医药导报》2017年第7期757-761,共5页Herald of Medicine

基　　金：乐清市科学技术局社会发展项目(2015Y001);浙江省大学生科技创新活动计划项目(2015R413011)

摘　　要：目的研究雷公藤多苷在同种异体小鼠胰岛移植中的抗免疫排斥作用。方法将20只C57BL/6小鼠制备糖尿病模型,肾被膜下移植Balb/c小鼠胰岛,随机分成雷公藤多苷组和模型对照组各10只。雷公藤多苷组腹腔注射雷公藤多苷溶液,模型对照组腹腔注射等量溶剂,均5 mg·kg^(-1)·d^(-1),连续14 d。移植4周内测定2组小鼠血糖和体质量;移植2周后,小鼠含胰岛肾组织行苏木精-伊红(HE)染色、胰岛素免疫组化染色,Western blotting检测移植物白细胞介素(IL)-2蛋白表达水平。结果 2组小鼠经胰岛肾被膜下移植后血糖均降至正常,模型对照组2周后血糖逐渐上升。雷公藤多苷组移植物炎症细胞浸润少,且胰岛素免疫组化染色较深;模型对照组炎症细胞大量浸润,胰岛素免疫组化染色较浅。雷公藤多苷组IL-2表达量显著减少(P<0.05)。结论雷公藤多苷可显著减少受体对同种异体胰岛移植物的炎症细胞浸润和炎症因子表达,降低免疫排斥反应,提高移植物存活时间。Objective To investigate the effect of tripterygium glycosides on the resistance to immune rejection after allogeneic islet transplantation in mice. Methods Twenty C57BL/6 mice were treated with STZ diabetes mellitus and transplanted the islets from Balb/c mice donor,then recipient mice were randomly divided into two groups：triptolide group and model control group（n=10）,and were intraperitoneal injected with tripterygium glycoside solutin and equivalent solvent of 5 mg·kg^-1·d^-1 for 14 days.Blood glucose and body weight were measured within 4 weeks after transplantation.Two weeks later,two groups of grafted islets were stained by HE staining and immunohistochemical staining,the expression of IL-2 protein were detected by Western blotting. Results The level of blood glucose were decreased to normal in the triptolide group and model control group after islet transplantation,but blood glucose gradually increased in the model control group after two weeks.Compared with the model control group,the inflammatory cells were less infiltrated and the immunohistochemical staining of insulin was deeper in the triptolide group.The expression of IL-2 in the triptolide group was significantly decreased（P〈0.05）. Conclusion Tripterygium glucoside could significantly decrease the inflammatory cell infiltration and inflammation factor expression in the allogeneic islet recipients to reduce the immune rejection and improve graft survival.

关 键 词：雷公藤多苷 胰岛移植 免疫排斥 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]