正交试验结合HPLC指纹图谱优选丹参酮类提取物的工艺  

Optimization of Extraction Technology for Tanshnones by Orthogonal Test Design Combining HPLC Fignerprint

作　　者：李国转 彭代银[1] 陈卫东[1] 邱镇[1] 于凡[1] 姚亮[1] 

机构地区：[1]安徽中医药大学,合肥230012

出　　处：《江西中医药大学学报》2017年第4期67-70,100,共5页Journal of Jiangxi University of Chinese Medicine

基　　金：安徽省教育厅2016-2018年高校科研创新平台团队(皖教秘科[2015]49号);安徽道地中药材品质提升协同创新中心项目(皖教科[2013]2号);国家基本药物所需中药材种子种苗安徽繁育基地建设项目(国中医药办规财发[2013]45号)

摘　　要：目的:优化丹参酮类提取物的工艺。方法:应用HPLC指纹图谱技术,以三个主要成分的含量(隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA)为次要指标,以HPLC特有峰总面积和干浸膏得率为主要评价指标,选取料液比、提取时间和乙醇浓度三个因素,采用L9(34)正交试验来确定最适合的工艺。结果:最佳提取方法:以8倍量的95%的乙醇为溶剂,提取3次,一次0.5 h。结论:优选得到的丹参酮类提取物的提取工艺稳定可行,不仅为丹参酮的产业化的提取提供了可借鉴的经验,而且为丹参相关制剂的进一步开发利用提供了理论依据。Objective： To optimize the extraction process and condition of tanshnones. Methods： Using high performance liquid chroma-tography （HPLC） fingerprint technology, the effects of solid - liquid ratio, ethanol concentration, extraction time were evaluated by or-thogonal test design with multi - index of content of the three monomer component （ cryptotanshinone, tanshinone I , tanshinone II A） that as the secondary indicators, the total area of characteristic peaks of HPLC and the yield of powdered extract that as the main index. Results： The optimum condition of the extraction technology was obtained. Solid - liquid ratio was 1 ： 8 μm/L , the ethanol concentration was 95% , the extraction time was 0. 5h, three times. Conclusion： This experiment has established the stable and feasible extraction technology of tanshnones. It not only provides reference experience for the extraction of tanshinones, but also provides a theoretical ba-sis for further development and utilization of S a lv ia miltiorrhiza Bge.

关 键 词：丹参酮类提取物 提取工艺 HPLC指纹图谱 正交试验 

分 类 号：R284.1[医药卫生—中药学]