苯并咪唑衍生物BMT-1对Jurkat细胞增殖及凋亡的影响  

The Effect of Benzimidazole Derivative BMT-1 on the Proliferation and Apoptosis of Jurkat Cells

作　　者：樊苗[1] 孙海[1] 唐信威[1] 赵波波[1] 欧阳寒梅[1] 邹强[2] 

机构地区：[1]成都医学院基础医学院,成都610500 [2]成都医学院科研实验中心,成都610500

出　　处：《成都医学院学报》2016年第5期528-531,共4页Journal of Chengdu Medical College

基　　金：国家自然科学基金项目(No:81273530)

摘　　要：目的探讨苯并咪唑衍生物BMT-1对急性T淋巴细胞白血病Jurkat细胞增殖及凋亡的影响。方法采用CCK-8测定BMT-1对Jurkat细胞增殖的影响,采用流式细胞术检测BMT-1对Jurkat细胞的细胞周期、细胞凋亡和线粒体膜电位的影响。结果 BMT-1可显著抑制Jurkat细胞增殖,呈剂量依赖效应,GI50为1.08μmol/L。BMT-1可诱导Jurkat细胞SubG1峰显著增强、凋亡细胞比例显著增加和线粒体膜电位显著改变。结论苯并咪唑衍生物BMT-1能显著抑制Jurkat细胞增殖,并诱导细胞凋亡。Objective To explore the effect of benzimidazole derivative BMT-1 on the proliferation and apoptosis of Jurkat cells.Methods CCK-8 was used to detect the effect of BMT-1 on the proliferation of Jurkat cells,and the flow cytometer was adopted to measure the effect of BMT-1 on the cell cycle,cell apoptosis and mitochondrial membrane potential of Jurkat cells.Results BMT-1 strongly inhibited the growth of Jurkat cell with a GI50 value of 1.08 μmol/L in dose-dependent manner.It induced the enhancement of SubG1 phase of Jurkat cells,elevated the proportion of apoptosis,and changed the mitochondrial membrane potential of Jurkat cells greatly.Conclusion Benzimidazole derivative BMT-1 could significantly inhibit proliferation and induce apoptosis in Jurkat cells.

关 键 词：苯并咪唑衍生物 JURKAT细胞 细胞凋亡 

分 类 号：R96[医药卫生—药理学]