CASP3和CASP7基因多态性与噪声性听力损失易感性关系  

Association of CASP3 and CASP7 gene hpolymorphism with susceptibility of noise-induced hearing loss in Chinese Han workers

作　　者：齐明键 高鹤[1] 曹承建[2] 邵玉仙[2] 谭晓华 张进霞[1] 吴茵茵 杨磊[1,3] 

机构地区：[1]石河子大学医学院预防医学教研室,新疆石河子832003 [2]杭州市职业病防治院 [3]杭州师范大学医学院

出　　处：《中国公共卫生》2017年第6期905-911,共7页Chinese Journal of Public Health

基　　金：浙江省重点科技创新团队项目(2011R50021);杭州市卫生科技计划一般项目(2014A70)

摘　　要：目的探讨细胞凋亡通路相关基因CASP3和CASP7多态性与中国汉族人群噪声性听力损失(NIHL)易感性之间的关联。方法研究对象来自2014年杭州市1 549名噪声接触工人听力损失的横断面调查,采用1:1配对病例对照研究,病例组为电测听双耳高频平均听阈>25 d B(A)的工人,对照组为性别、年龄、接噪工龄、工作岗位与病例匹配且双耳所有频段听阈均≤25 d B(A)的工人,共272对。PCR-LDR法检测2个SNP位点的基因型。采用多因素条件logistic回归模型分析SNP位点与NIHL的关联,并以叉生分析计算基因-环境交互作用。结果χ~2检验分析发现,CASP3基因rs1049216等位基因(C、T)频率组间分布有统计学差异(OR=0.68,95%CI=0.50~0.93),而基因型频率组间差异无统计学意义(P>0.05);CASP7基因rs10787498等位基因及基因型频率组间分布均无统计学意义(P>0.05);多因素条件logistic回归显示,CASP3基因rs1049216中,与野生基因型CC相比,突变基因型(CT+TT)为NIHL的保护因素(调整OR=0.65,95%CI=0.43~0.97);叉生分析表明rs1049216位点与文化程度、睡眠时间存在交互作用(P≤0.001),NIHL的危险性降低(OR值变小)。结论 CASP3基因rs1049216位点可能与中国汉族人群NIHL易感性有关,且可能与文化程度、睡眠时间存在交互作用。Objective To investigate the association between CASP3 and CASP7 gene polymorphism and the susceptibility of noise-induced hearing loss （NIHL） in a Chinese Han occupation population.Methods A 1：1 case-control study was conducted among 272 cases with high frequency hearing threshold of 〉25 decibels（dB）and 272 gender-,age-,years of noise exposure-,and workplace-matched controls with the threshold of ≦25dB selected from a total of 1 549 noise-exposed workers in Hangzhou city.The genotypes of CASP3 rs1049216 and CASP7 rs10787498 were determined with polymerase chain reaction-ligase detection reaction（PCR-LDR）.The associations of genetic variations of CASP3 and CASP7 with NIHL were assessed by using multivariate logistic regression,and dichotomy analysis was used to evaluate the interaction between environmental risk factors and gene polymorphism on HIHL.Results There was no statistical difference in the frequency of alleles and genotypes of CASP7 rs10787498 between cases and controls.But genetic variation of CASP3 rs1049216 was associated with NIHL;the subjects with mutant allele （CT or TT） of CASP3 rs1049216 showed a decreased NIHL risk （adjusted odds ratio [OR]=0.65,95% confidence interval [95%CI]：0.43-0.97）.The results of dichotomy analysis revealed interactions between genotype of CASP3 rs1049216 and environment risk factors including education level and sleeping time.Conclusion There is a correlation between CASP3 rs1049216 mutation and the susceptibility of NIHL and there are interactions between the influences of CASP3 rs1049216 polymorphism and educational level and sleeping time on the susceptibility of NIHL in Chinese Han workers.

关 键 词：CASP3 CASP7 噪声性听力损失 单核苷酸多态性 

分 类 号：R764.433[医药卫生—耳鼻咽喉科]