天门冬对氟中毒大鼠学习记忆能力干预作用  

Intervention effect of Radix asparagi on learning and memory in fluorosis rats

作　　者：刘洋[1] 李艳菊[2] 李红日[1] 王宁 刘振华[4] 周媛[1] 王飞清 

机构地区：[1]贵阳中医学院第一附属医院检验科,贵州贵阳55001 [2]贵州医科大学附属医院 [3]贵州省骨科医院 [4]贵州省人民医院

出　　处：《中国公共卫生》2017年第6期922-925,共4页Chinese Journal of Public Health

基　　金：贵州省科技厅项目(黔科合LH字[2016]7511号;黔科合基础[2016]1019);贵州省教育厅青年科技人才成长项目(黔教合KY[2016]190)

摘　　要：目的探讨天门冬对氟中毒致大鼠学习记忆能力障碍干预作用。方法 60只SD大鼠随机分为5组,每组12只,分别为对照组、染氟组、天门冬低、中、高剂量组(3、6、12 g/kg),于染氟90 d处死大鼠;观察染氟期间大鼠氟斑牙情况,采用氟离子选择电极法检测大鼠尿氟和骨氟含量,Morris水迷宫和跳台实验检测大鼠学习记忆能力。结果染氟组大鼠氟斑牙发生率、尿氟和骨氟含量明显高于对照组;与染氟组比较,各剂量天门冬组大鼠中、重度氟斑牙发生率、骨氟含量明显降低,尿氟含量明显升高,呈剂量效应关系。与对照组比较,染氟组大鼠逃避潜伏期明显延长[(31.67±8.80)s](P<0.05);与模型组比较,中、高剂量天门冬组大鼠逃避潜伏期[分别为(17.67±6.62)、(14.33±4.13)s]明显缩短(P<0.05)。与对照组比较,染氟组大鼠穿越平台次数[(1.33±1.03)次]下降(P<0.05);与染氟组比较,中、高剂量天门冬组穿越平台次数[分别为(2.67±2.16)、(4.17±1.47)次]明显增加(P<0.05)。与对照组比较,染氟组大鼠跳台实验反应时间[(101.33±22.47)s]明显延长,潜伏时间[(104.33±30.22)s]明显缩短(P<0.05);与染氟组比较,中、高剂量天门冬组大鼠跳台实验反应时间[分别为(74.67±18.83)、(55.33±12.47)s]明显缩短,潜伏时间[分别为(135.33±26.55)、(156.67±28.35)s]明显延长(P<0.05)。结论氟中毒可影响大鼠学习记忆能力,给予不同剂量天门冬干预,可改善大鼠学习记忆能力。Objective To investigate the intervention effect of Radix asparagi on learning and memory impairment induced by fluoride poisoning in rats.Methods Sixty Sprague-Dawley（SD）rats were randomly divided into 5 groups（12 in each group）：a control group with basal feed,a fluoride group with basal feed and 50 mg/kg sodium fluoride,and low-,moderate-,and high-dose Radix asparagi group with feed and 50 mg/kg sodium fluoride and administered with Radix asparagi decoction at dosages of 3,6,and 12 g/kg by gavage once a day continuously for 90 days.Dental fluorosis of the rats was observed during the treatment.By the end of the treatment,Morris water maze and platform experiment testing were performed to assess the ability of learning and memory of the rats and urine and bone fluoride of the rats were detected with fluorine ion selective electrode method.Results The incidence of dental fluorosis and contents of urine and bone fluoride of the rats exposed to fluoride were significantly higher than those of the control rats.Compared with those in the rats of the fluoride group,the incidence of moderate and severe dental fluorosis and bone fluoride content decreased significantly while the urine fluoride content increased significantly in a dose-dependent manner in the rats of Radix asparagi groups.Compared with the rats of the control group,the fluoride-exposed rats showed a significantly increased escape latency of 31.67±8.80 s （P〈0.05）;compared with the rats of fluoride group,the rats administered with moderate-and high-dose Radix asparagi demonstrated significantly reduced escape latencies of 17.67±6.62 s and 14.33±4.13 s （both P〈0.05）.Compared with the rats of the control group,the fluoride-exposed rats showed a significantly decreased time of across the platform of 1.33±1.03 （P〈0.05）;compared with the rats of fluoride group,the rats administered with moderate-and high-dose Radix asparagi demonstrated significantly increased times of across the platform of 2.67±2.16 and 4.17±1.47 （both P〈

关 键 词：天门冬 氟中毒 干预 学习记忆 

分 类 号：R994.6[医药卫生—毒理学]