槲皮素对人胃癌MKN45细胞的抑制作用研究  

Inhibition of quercetin on human gastric carcinoma MKN45 cells

作　　者：付丽娜[1] 刘维红[1] 徐爱军[1] 宋丽杰[1] 

机构地区：[1]天津市第四中心医院消化内科,天津300140

出　　处：《现代药物与临床》2017年第6期983-986,共4页Drugs & Clinic

基　　金：天津市中医中西医结合科研课题(2015054)

摘　　要：目的探讨槲皮素对人胃癌MKN45细胞凋亡的影响及其作用机制。方法取对数生长期的MKN45细胞分为对照组和20、40、80μmol/L槲皮素组。应用MTT比色法测定细胞活性,Hoechst染色、流式细胞技术检测细胞凋亡,比色法检测凋亡相关因子Caspase-3、Caspase-9表达。结果槲皮素能显著抑制MKN45细胞增殖,促进细胞凋亡,升高Caspase-3、Caspase-9基因表达水平。结论槲皮素可通过增强Caspase-3、Caspase-9基因表达诱导人胃癌MKN45细胞凋亡。Objective To investigate the effect and mechanism of quercetin on MKN45 cells. Methods MKN45 cells were divided into four group： negative control group and quercetin groups （20, 40, and 80 μmol/L）. Cell viability was measured by MTT colorimetric assay, apoptosis was detected by Hoechst staining and flow cytometry assay, and expression of apoptosis-related factors Caspase-3 and Caspase-9 were tested by colorimetric detection. Results Quercetin could obviously inhibit MKN45 cell proliferation and promote apoptosis, and could increase Caspase-3 and Caspase-9 gene expression levels. Conclusion Quercetin can induce gastric carcinoma MKN45 cells apoptosis by enhancing the expression of Caspase-3 and Caspase-9 gene.

关 键 词：槲皮素 MKN45细胞 凋亡 CASPASE-3 CASPASE-9 

分 类 号：R966[医药卫生—药理学]