运动营养品中磺胺类成分的残留检测  

Determination of Sulfanilamide Residue in Sports Nutrition

作　　者：陶弥锋[1] TAO Mi-feng(Department of Sports, Jiangxi University of Technology, Nanchang 330029, Jiangxi, Chin)

机构地区：[1]江西科技学院体育部,江西南昌330029

出　　处：《食品研究与开发》2017年第13期165-168,共4页Food Research and Development

摘　　要：建立一种用高效液相色谱法测定运动营养品中9种磺胺类成分残留的检测方法。样品经乙醇提取后,过固相萃取柱后上机检测。采用ZORBAX SB C_(18)分析色谱柱分离,以甲醇-1.0%甲酸作为流动相的梯度洗脱模式下,外标法峰面积定量。结果表明,9种组分在0.05μg/mL^10.00μg/mL浓度范围内线性良好,相关系数r^2为0.999 0~0.999 8,检出限在10μg/kg^15μg/kg,定量限在30μg/kg^45μg/kg,加标回收率达到79.2%~84.7%。A rapid and effective method was established for the determination of nine kinds of sulfanilamide residue in sports nutrition by high performance liquid chromatography method. The samples were extracted by ethanol,and detected after purified by solid phase extraction column. The separation of target compound was performed on a ZORBAX SB C18 chromatography column using methanol-1.0%formic acid as mobile phase by the gradient elution mode, and external standard method peak area quantification. The results show that the lin-ear range of nine kinds of sulfanilamide was in the range of 0.05μg/mL-10.00μg/mL with a correlation coeffi-cient of 0.9990-0.9998. The detection limit was 10μg/kg-15μg/kg and the quantitative limit was 30μg/kg-45μg/kg. The recovery rate was 79.2%-84.7%.

关 键 词：高效液相色谱 运动营养品 磺胺类药物 

分 类 号：O657.72[理学—分析化学] TS218[理学—化学]