UPLC-MS/MS检测乳品饮料中的四环素类残留  

Analysis Tetracycline Antibiotics Residues in Dairy Beverage by UPLC-MS/MS

作　　者：李婷婷[1] 韦曙光 彭艳[1] LI Ting-ting WEI Shu-guang PENG Yan(School of Chemistry and Eco-Environmental Engineering（School of Chinese Pharmacy ）, Guizhou Minzu University, Guiyang 550025, Guizhou, China Guizhou Survey ＆ Design Reserch Institute for Water Resources and Hydropower, Guiyang 550001, Guizhou, China)

机构地区：[1]贵州民族大学化学与生态环境工程学院(民族医药学院),贵州贵阳550025 [2]贵州省水利水电勘测设计研究院,贵州贵阳550001

出　　处：《食品研究与开发》2017年第13期182-185,共4页Food Research and Development

摘　　要：建立一种超高效液相色谱-串联质谱法检测乳品饮料中四环素、土霉素、金霉素、强力霉素残留的分析方法。样品前处理用乙腈沉淀蛋白后,采用HLB小柱进行净化和富集。采用Waters Atalantis T3色谱柱(2.1 mm×150 mm,3μm)分离,以0.1%甲酸-乙腈为流动相的梯度洗脱模式下,4种四环素类抗生素在1.0 ng/mL^200.0 ng/mL浓度范围内线性良好,相关系数r^2为0.998 5~0.999 2,检出限为0.5μg/kg^2.0μg/kg,定量限为1.5μg/kg^6.0μg/kg,加标回收率达到82.5%~91.5%。A ultra high performance liquid chromatography mass spectrometry analysis method was established for the residues determination of tetracycline, oxytetracycline, chlortetracycline and doxycycline in dairy bev-erage. After the sample was treated with acetonitrile, it was concentrated and purified by HLB solid phase ex-traction column. The separation of target compound was performed on a Waters Atalantis T3 chromatographic column（2.1 mm × 150 mm, 3μm） using 0.1%formic acid-acetonitrile as mobile phase under gradient elution mode. The linear range of 4 tetracycline antibiotics components was in the range of 1.0 ng/mL-200.0 ng/mL with a correlation coefficient of 0.9985-0.9992. The detection limit was 0.5μg/kg-2.0μg/kg and the quantitative limit was 1.5μg/kg-6.0μg/kg. The recovery rate was 82.5%-91.5%.

关 键 词：超高效液相色谱-串联质谱 乳品饮料 四环素类 

分 类 号：O657.63[理学—分析化学] TS275.4[理学—化学]