萝卜细胞质雄性不育的分子鉴定及orf138/orfB基因序列差异性分析  

Molecular Identification of Cytoplasmic Male Sterility in Radish( Raphanus sativus L. ) and Sequence Analysis of orf138 and orf B

作　　者：李晓梅[1] 柴靓[2] 杨峰[1] 冉茂林[1,3] LI Xiao-mei CHAI Liang YANG Feng RAN Mao-lin(Institute of Rice and Sorghum, Sichuan Academy of Agricultural Sciences, Siehuan Deyang 618000, China Institute of Crop Re- search, Siehuan Academy of Agricultural Sciences, Sichuan Chengdu 610066, China Sichuan Province Key Lak~oratory of Vegetable Germplasm and Varieties Innovation, Siehuan Chengdu 610066, China)

机构地区：[1]四川省农业科学院水稻高粱研究所,四川德阳618000 [2]四川省农业科学院作物研究所,四川成都610066 [3]蔬菜种质与品种创新四川省重点实验室,四川成都610066

出　　处：《西南农业学报》2017年第6期1262-1267,共6页Southwest China Journal of Agricultural Sciences

基　　金：四川省科学技术厅"四川省科技创新苗子工程"(2016092)

摘　　要：【目的】本研究旨在了解萝卜雄性不育细胞质的分子特征。【方法】以8份萝卜细胞质雄性不育系及其保持系、5份不育材料及6个萝卜品种为试材,利用线粒体基因orf138及orf B为分子标记,设计特异引物对材料进行扩增,并对扩增产物进行测序分析。【结果】8份萝卜细胞质雄性不育系、5份不育材料及5个萝卜品种检测到含有orf138特异片段,为Ogura胞质类型,不育系相应保持系则未检测到特异片段;对扩增产物测序发现所有Ogura胞质材料可分为2种类型,Type A和Type H;27份材料orf B特异扩增后,其产物按编码区核苷酸序列的差异可分为2个类型,Ogura胞质中序列一致,普通胞质中序列一致。【结论】由此可见,orf138与orf B基因在Ogura胞质与普遍胞质间均差异明显,可利用orf138的有无及orf B基因的核苷酸序列差异进行胞质类型鉴定。[ Objective] The present study was conducted to understand the molecular characteristics of male-sterile cytoplasm in radish. [ Method] The orf138 and orfB of mitochondrial loci were used as molecular markers, and a total of eight male-sterile lines, eight maintainer lines, five male-sterile populations and six cultivars by PCR amplification were analyzed. [ Result ] The orf138 gene of the eight male-sterile lines, five male-sterile populations and five cuhivars by specific amplification were Ogura CMS, but their maintainer lines d：~! ~：~t. Sequence analysis of orf138 demonstrated that the Ogura CMS could be divided into two types, type A and type H. Based on the mutations of the cod- ing region sequences of orfB, two types of orfB were found in total of 27 tested materials, and the sequences from Ogura and normal cyto- plasm were absolutely distinguished. [ Conclusion] Thus, the genes of orf138 and orfB between the Ogura cytoplasm and the general cyto- plasm were significantly different, and the presence of orf138 and the nucleotide sequence differences of orfB would be used in cytoplasm type identification.

关 键 词：萝卜(Raphanus SATIVUS L.) 雄性不育细胞质 分子鉴定 ORF138 orf B 

分 类 号：S631.1[农业科学—蔬菜学]