紫果西番莲炭疽病病原菌鉴定及生物学特性研究  

Study on Identification and Biological Characteristics of Anthracnose Pathogen of Passiflora edulis

作　　者：谢美华[1,2,3] 杨金奎 李雪玲[1,3] 杨海艳[1,3] 陈莉莉[1] 吴帆[1] XIE Mei-hua YANG Jiu-kui LI Xne-ling YANG Hai-yan CHEN Li-li WU Fan(Department of Chemistry and Life Science, Chuxiong Normal University, Yunnan Chuxiong 675000, China Laboratory for Conserva- tion and Utilization of Bio-Resources, Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Yunnan Kunming 650091, China Yunnan Province Applied Biology Key Laboratory of University, Yunnan Chuxiong 675000, China)

机构地区：[1]楚雄师范学院化学与生命科学系,云南楚雄675000 [2]省部共建云南生物资源保护与利用国家重点实验室,云南昆明650091 [3]云南省高校应用生物学重点实验室,云南楚雄675000

出　　处：《西南农业学报》2017年第6期1358-1363,共6页Southwest China Journal of Agricultural Sciences

基　　金：楚雄师范学院后备人才资助项目(XJRC1502);云南省重点建设学科;楚雄师范学院重点建设学科基金项目(05YJJSXK03);云南省高校科技创新团队支持计划项目(IRTSTYN);云南省教育厅项目(2014Y443);云南省教育厅科学研究基金项目(2017ZZX016)

摘　　要：【目的】对紫果西番莲叶片炭疽病病原X-03进行分离鉴定,并对其生物学特性和杀菌剂对X-03的抑制作用进行研究。【方法】采用形态观察与分子生物学相结合的方法。【结果】X-03为刺盘孢属(Colletotrichum sp.)真菌,它的最适生长温度为15~20℃,最适生长p H为p H 8~9,当温度高于50℃时X-03菌株的孢子不能萌发。X-03生长最适培养基为PDA,最适碳源和氮源分别为葡萄糖和Na NO3。供试的杀菌剂中以福美双对X-03的抑制效果最好。【结论】研究可为紫果西番莲炭疽病的防治提供理论依据。[ Objective ] The pathogen （ X-03 ） of plant a.nthracnosis was identified from Passiflora edulis, and it＇s biological characteristics andinhibition effect of four fungicides on X-03 were investigated. [ Method ] Morphologic observation combined with molecular biological tech-niques were employed in the paper. [ Result ] The results showed that X-03 belonged to genus Colletotrichum, and the optimal growth condi-tion for this fungus was 15 -20 ℃ and pH 8 -9, while the spores could not germinate when the temperature over 50 ℃. The most suitablemedium was PDA for X-03, and the optimal C and N sources were glucose and NaNO3 , respectively. The inhibitory effect of Thiram on X-03 was more obvious than other tested germicides. [ Conclusion ] This study could provide a theoretical basis for the prevention and control ofanthraenose of Pass（flora edulis.

关 键 词：紫果西番莲 炭疽病 病原 生物学性状 

分 类 号：S667.9[农业科学—果树学]