跑台运动干预膝关节软骨全层缺损修复  

Effect of treadmill exercise on the repair of full-thickness knee cartilage defects

作　　者：赵森泉 毛新润 Zhao Sen-quan Mao Xin-run(Hebei Software Institute, Baoding 071000, Hebei Province, China the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China)

机构地区：[1]河北软件职业技术学院,河北省保定市071000 [2]河北医科大学第二附属医院,河北省石家庄市050000

出　　处：《中国组织工程研究》2017年第16期2478-2483,共6页Chinese Journal of Tissue Engineering Research

基　　金：2016河北省医学科学研究重点课题计划项目(20160122)~~

摘　　要：背景:关节软骨位置特殊,其功能常会受到运动的影响,通过关节运动能促进缺损部位修复,但是该结论尚未得到进一步证实。目的:探讨跑台运动时不同运动强度对大鼠膝关节软骨全层缺损修复效果。方法:将40只大鼠用直径3.2 mm的骨科手术电钻在股骨面制作一个直径3.2 mm的全层软骨缺损模型,然后随机分为模型组、低强度运动组、中强度运动组和高强度运动组,各10只。模型组仅在笼内自由活动,低强度运动组在平面进行运动,平台速度8.2 m/min;中强度运动组跑台坡度设置成5°,速度控制在15.5 m/min;高强度运动组设置跑台高度10°,速度控制在26.7 m/min。结果与结论:(1)跑台运动8周后,低、中、高强度运动强度组大鼠血清基质金属蛋白酶3、基质金属蛋白酶抑制剂浓度均低于模型组(P<0.05),且中强度运动组大鼠血清基质金属蛋白酶3、基质金属蛋白酶抑制剂浓度低于低、高强度运动强度组(P<0.05);(2)中强度运动组关节软骨全层缺损区可见纤维组织,且与相邻正常关节软骨连接,厚度基本,修复效果明显优于其他3组。(3)提示中等强度跑台运动能促进大鼠膝关节软骨全层缺损修复,提高关节功能。BACKGROUND： As the articular cartilage has a special anatomical position, its function is often affected by movement. Whether exercise training can promote the cartilage repair still needs to be confirmed. OBJECTIVE： To investigate the effects of different intensities of treadmill exercises on the repair of full-thickness articular cartilage defects in rats. METHODS： Forty rats were selected, and the models of full-thickness articular cartilage defects （3.2 mm in diameter） on the femoral surface were made with a orthopedic drill, followed by randomized into model, low-, moderate- and high-intensity exercise groups （n=10 per group）. The rats in the model group were allowed free cage activity. The rats in the low-intensity exercise group exercised on the platform, and the platform speed was 8.2 m/min; rats in the middle-intensity exercise group exercised on the 5° treadmill, and the speed was controlled at 15.5 m/min; rats in the high-intensity exercise group exercised on the 10° treadmill, and the speed was controlled at 26.7 m/min. RESULTS AND CONCLUSION： The levels of serum matrix metalloproteinase-3 and inhibitor of matrix metalloproteinase in the latter three groups were significantly lower than those in the model group at 8 weeks after exercise （P 〈 0.05）, and the level was the lowest in the moderate-intensity exercise group （P 〈 0.05）. In the moderate-intensity exercise group, there were fibrous tissues in the defect area, which were connected with the adjacent cartilage, and the repair effect was superior to that in the other three groups. To conclude, moderate-intensity treadmill exercise can promote the repair of full-thickness knee cartilage defects in rats, thereby improving functional recovery of the rat knee joint.

关 键 词：软骨细胞 膝关节 软骨 关节 组织工程 组织构建 软骨组织工程 软骨缺损 跑台运动 膝关节软骨 基质金属蛋白酶3 关节功能 全层软骨缺损模型 纤维细胞 

分 类 号：R318[医药卫生—生物医学工程]