不同强度冲击波对牙槽骨细胞成骨能力的影响  

Influence of different intensities of extracorporeal shock waves on the osteogenesis ability of alveolar osteoblasts

作　　者：郭元[1] 张弛[2] 刘松[2] 王钊[2] 潘兴飞[3] 王簕[2] Guo Yuan Zhang Chi Liu Song Wang Zhao Pan Xing-fei Wang Le(Department of Stomatology Department of Orthopedics Department of Infectious Diseases, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, China)

机构地区：[1]广州医科大学附属第三医院口腔科,广东省广州市510150 [2]广州医科大学附属第三医院骨科,广东省广州市510150 [3]广州医科大学附属第三医院感染科,广东省广州市510150

出　　处：《中国组织工程研究》2017年第16期2509-2514,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(31570980);广东省公益研究与能力建设项目(2014A020212347);广州市属高校科研项目(1201430092)~~

摘　　要：背景:许多口腔炎性疾病可引起牙槽骨丢失,牙齿松动,影响咬合关系。体外冲击波技术有望改善牙槽骨修复,提高牙槽骨细胞成骨活性,但是目前体外冲击波技术对牙槽骨细胞的作用效果及机制仍不明确,有必要进一步阐明。目的:比较不同强度冲击波对大鼠牙槽骨细胞增殖能力和成骨分化的影响。方法:通过原代培养获取大鼠牙槽骨细胞,进行体外分离培养,通过碱性磷酸酶染色进行鉴定。分别用强度为0.18、0.36、0.50 mJ/mm^2的冲击波作用于大鼠牙槽骨细胞,作用频次均为100次。结果与结论:当选用强度为0.18和0.36 mJ/mm^2冲击波作用后,牙槽骨细胞碱性磷酸酶和骨形态发生蛋白2的分泌量明显增加,并且冲击波强度越高,分泌量越大(P<0.05)。当冲击波作用强度为0.50 mJ/mm^2时,大鼠牙槽骨细胞增殖能力明显减退,碱性磷酸酶和骨形态发生蛋白2的分泌量显著下降(P<0.05)。提示当冲击波作用强度低于0.36 mJ/mm^2时,随着作用强度的增加,可提高牙槽骨细胞的成骨能力,这为临床应用冲击波促进牙槽骨修复提供了理论基础。BACKGROUND： Oral inflammatory diseases usually cause alveolar bone loss and odontoseisis, and further impact dental occlusion. Extracorporeal shock wave therapy （ESWT） is a promising method for the repair of alveolar bone and improving osteogenic activity of alveolar osteoblasts, but its therapeutic efficacy and related mechanisms remain unclear. OBJECTIVE： To compare the effect of different intensities of ESWT on the proliferation and osteogenesis abilities of rat alveolar osteoblasts. METHODS： The rat alveolar osteoblasts were obtained and cultured in vitro, and further identified by alkaline phosphatase staining. 0.18, 0.36, and 0.50 mJ/mm^2 ESWT was used to stimulate the rat alveolar osteoblasts, 100 pulses, respectively. RESULTS AND CONCLUSION： The levels of alkaline phosphatase and bone morphogenetic protein 2 were significantly increased in the 0.36 and 0.18 mJ/mm^2 ESWT groups, especially in the 0.36 mJ/mm^2 ESWT group （P 〈 0.05）. 0.50 mJ/mm^2 ESWT significantly decreased the proliferation ability of rat alveolar osteoblasts and downregulated the levels of alkaline phosphatase and bone morphogenetic protein 2 （P 〈 0.05）. To conclude, ESWT （〈 0.36 mJ/mm^2） can improve the osteogenesis ability of rat alveolar osteoblasts with the intensity increasing, which provides a theoretical basis for the clinical use of ESWT in the alveolar bone repair.

关 键 词：高能量冲击波 细胞分化 组织工程 组织构建 骨细胞 冲击波 牙槽骨细胞 成骨 碱性磷酸酶 骨形态发生蛋白2 骨修复 细胞增殖 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]