流动剪切力促小口径组织工程血管内皮化  

Flow shear stress promotes the endothelialization of small diameter tissue-engineered vessels

作　　者：张伟 宋爱晶 Zhang Wei Song Ai-jing(Guanhao Biotech Co., Ltd., National Engineering Laboratory for Regenerative and Implantable Medical Devices, Guangzhou 510530, Guangdong Province, China Guangdong Provincial Institute of Sport Science, Guangzhou 510663, Guangdong Province, China)

机构地区：[1]冠昊生物科技股份有限公司再生型医用植入器械国家工程实验室,广东省广州市510530 [2]广东省体育科学研究所,广东省广州市510663

出　　处：《中国组织工程研究》2017年第16期2515-2520,共6页Chinese Journal of Tissue Engineering Research

基　　金：广东省重大科技专项(2010A080407005);国家重点研究计划生物医用材料研发与组织器官修复替代重点专项(2016YFC1103202)~~

摘　　要：背景:细胞内皮化的流动剪切力大小和作用方式是小口径组织工程血管的构建中的关键问题。小口径组织工程血管构建后的抗血流冲击能力是决定移植成功的关键因素之一,较多人把研究重点集中在如何制作移植血管支架材料,如何提高宿主细胞种植后其血管内壁的抗凝效果,而对细胞内皮层的力学驯化则关注较少,该方法有可能是提高组织工程血管移植成功率的有效手段之一。目的:比较单一水平和成梯度增加的流动剪切力对组织工程血管内皮化的影响,获得适用于组织工程血管内皮化的剪切力大小和加载方式。方法:采用血管铺片硝酸银染色法和内皮细胞肌动蛋白荧光标记法,观察组织工程血管内壁上内皮细胞的数量、形状和黏附性能等变化情况。结果与结论:经2.5和3.0 N/m^2剪切力作用2 h后,血管内壁上的内皮细胞几乎全部脱落;而经0.5-3.0 N/m^2,每2 h增加0.2 N/m^2的成梯度增加的剪切力作用后,血管内皮细胞层保持较完整,细胞形态成明显的轴向排列,且能诱导内皮细胞中应力纤维的出现。说明成梯度增加的流动剪切力这种"驯化"方式,能较好地促进小口径组织工程血管的内皮化。BACKGROUND： Magnitude and action ways of flow shear stress are important for the endothelialization of small diameter tissue-engineered vessels （TEV）, and the TEV resistance ability to blood flow even decides the destiny of implantation. There are many studies on how to construct the TEV and improve anticoagulant ability of TEV after host cell implantation, but the effects of different flow shear stresses on the TEV endothelialization is rarely reported, which may be helpful for increasing the success rate of TEV implantation. OBJECTIVE： To compare the effects of flow shear stresses in single level or stepwise increased on the endothelialization of small diameter TEV and to optimize the TEV in the aspects of shear stress magnitude and loading method. METHODS： The number, morphology and adhesion ability of endothelial cells on the inner wall of TEV were observed through silver nitrate and F-actin staining. RESULTS AND CONCLUSION： Single-level shear stress at 2.5, 3.0 N/m^2 for 2 hours removed almost all the endothelial cells seeded on the inner wall of TEV. In contrast, stepwise increased shear stress from 0.5 N/m^2 to 3.0 N/m^2 at an increase of 0.2 N/m^2/2 hours maintained the integrity and oriented along the flow direction, and could induce stress fibers productionin endothelial cells. These results suggest that the stepwise increased flow shear stress can improve the endothelialization of TEV.

关 键 词：内皮细胞 生物力学 组织工程 组织构建 流动剪切力 小口径组织工程血管 内皮化 单一水平 成梯度增加 黏附性能 应力纤维 

分 类 号：R318[医药卫生—生物医学工程]