C促红细胞生成素在脑梗死后微循环再通中的作用  

Carbamylated erythropoietin promotes vascular microcirculation following cerebral infarction

作　　者：张起顺[1] 陈勇[1] 王朝辉[1] 吴春芳[1] 赵俊[1] Zhang Qi-shun Chen Yong Wang Zhao-hui Wu Chun-fang Zhao Jun(Department of Neurology, Huaihe Hospital of Henan University, Kaifeng 475000, Henan Province, Chin)

机构地区：[1]河南大学淮河医院神经内科,河南省开封市475000

出　　处：《中国组织工程研究》2017年第16期2534-2539,共6页Chinese Journal of Tissue Engineering Research

基　　金：河南省教育厅自然科学研究计划项目(2013B206253)~~

摘　　要：背景:C促红细胞生成素可明显提升脑梗死患者预后,且促进心肌梗死后脑微循环再生。目的:探究C促红细胞生成素提升脑梗死患者预后的具体机制。方法:150只Wistar大鼠,随机取120只建立大鼠脑梗死动物模型后分为:诱导脑梗死组,诱导脑梗死组+C促红细胞生成素处理(浓度500,1 000,2 000 U/kg)组,另30只大鼠为空白对照。体外分离并培养脑梗死动物模型微循环血管内皮细胞,CCK8实验检测细胞增殖情况;Western blot检测与增殖相关基因(Ki67,p16)和血管内皮生长因子蛋白表达,RNA干扰技术选择性沉默血管内皮生长因子蛋白的表达,重复上述指标检测。结果与结论:(1)CCK8实验显示,随着C促红细胞生成素浓度的上升,微循环血管内皮细胞的增殖能力也同步上调;Western blot显示,增殖相关基因(Ki67,p16)和血管内皮生长因子蛋白表达水平也显著上调;(2)shR NA-VEFG干扰后,CCK8及Western blot实验显示,微循环血管内皮细胞的增殖能力及增殖相关基因(Ki67,p16)的表达未随C促红细胞生成素浓度的上调成正比;(3)结果提示,C促红细胞生成素通过上调血管内皮生长因子促进脑梗死动物模型微循环血管内皮细胞的增殖。BACKGROUND： Carbamylated erythropoietin （CEPO） cannot only remarkably promote the prognosis of cerebral infraction, but also improve the microcirculation. OBJECTIVE： To explore the underlying mechanism of CEPO promoting the microcirculation following cerebral infraction. METHODS： 150 Wistar rats were selected, and 120 rats were used for establishing the models of cerebral infarction, followed by allotted into four groups. The model rats were treated with 500, 1000 and 2000 u/kg CEPO as experimental groups, and those received no treatment as model group. The other 30 rats were as controls. Vascular endothelial cells were isolated and cultured in vitro, and the cell proliferation was detected by cell counting kit-8 assay. The expression levels of proliferation-related genes （Ki67 and p16） and vascular endothelial growth factor （VEGF） were detected using western blot assay. After selective silencing of VEGF through RNA interference, all above indicators were detected again. RESULTS AND CONCLUSION： Cell counting kit-8 assay results showed that the proliferation ability of vascular endothelial cells was increased with CEPO concentration increasing. Western blot assay results showed a significant upregulation of Ki67, p16 and VEGF. After shRNA-VEFG interference, these indicators had no positive correlation with the increased concentration of CEPO. Our findings indicate that CEPO can improve the proliferation of vascular endothelial cells in an animal model of cerebral infarction via upregulating the VEGF expression.

关 键 词：脑梗塞 微循环 血管内皮生长因子类 组织工程 组织构建 C促红细胞生成素 脑梗死 微循环血管内皮细胞 增殖 血管内皮生长因子 

分 类 号：R318[医药卫生—生物医学工程]