反复冻融同种异体跟腱重建前交叉韧带移植物的组织学特点  被引量:1

Histological characteristics of the reconstructed anterior cruciate ligament with repetitive freeze-thawing allogenic Achilles tendon

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作  者:田明[1] 余家阔[1] 吴延平[1] Tian Ming Yu Jia-kuo Wu Yan-ping(Institute of Sports Medicine, Third Hospital of Peking University, Beijing 100191, China)

机构地区:[1]北京大学第三医院运动医学研究所,北京市100191

出  处:《中国组织工程研究》2017年第16期2546-2551,共6页Chinese Journal of Tissue Engineering Research

摘  要:背景:在临床应用中,经常出现解冻后的同种异体移植物由于某种原因而不用的情况,这样的异体移植物是否可以回收冷冻即经历了反复冻融的过程后继续使用呢?反复冻融后的移植物在体内的病理学改变尚无报道。目的:观察反复冻融的同种异体跟腱重建兔前交叉韧带后移植物的组织学改变。方法:无菌条件下选取成年雄性新西兰白兔异体跟腱,经密封包装和60Co照射灭菌后,在-80℃和20℃反复冻融。选用24只成年雄性新西兰白兔行前交叉韧带重建,其中12只左、右膝分别用冻融1次(对照组)、2次的移植物,另外12只左、右膝分别用冻融3次、10次的移植物。分别于造模后6,12,24周取材。每一时段各组随机取3条重建的前交叉韧带,通过Modified Histology Grading Score评分,评估重建前交叉韧带的细胞形态、数量、基质染色强度、纤维软骨、新骨形成、腱骨愈合、软骨损伤程度等。结果与结论:(1)造模后6周冻融10次组的细胞形态、基质染色强度和总分明显高于其他组(P<0.05),其他各组的评分差异无显著性意义(P>0.05);(2)虽然造模后6周反复冻融10次同种异体跟腱重建的兔膝前交叉韧带的组织学评分高于冻融次数少组,但造模后12,24周不同反复冻融次数对重建前交叉韧带组织学评分的影响无明显差异;(3)实验仅从组织学的角度证明反复冻融多次的肌腱在术后早期组织学评分优于冻融次数较少的肌腱。BACKGROUND: Thawed allografts are usually discarded for various reasons. Whether these discarded allografts can be refrozen for later use and their histological changes in vivo have not been reported. OBJECTIVE: To investigate the histological characteristics of anterior cruciate ligament (ACL) reconstructed with the repetitive freeze-thawing allogenic Achilles tendon in New Zealand white rabbits. METHODS: Allogenic Achilles tendons were harvested from adult male New Zealand white rabbits, and were stored at -8 ℃ and thawed at 20 ℃ for 1, 2, 3 and 10 times, respectively, after sealed package and 60Co irradiation. Twenty-four adult male New Zealand white rabbits were enrolled: the left and right knees of 12 rabbits were respectively reconstructed with 1 (control group) and 2 times of freeze-thawing allogenic Achilles tendon, and another 12 rabbits underwent reconstruction with 3 and 10 times of freeze-thawing allogenic Achilles tendon, respectively. Three specimens from each group were evaluated with modified histology grading scores at 6, 12 and 24 weeks to assess the cell morphology, cell quantity, matrix staining intensity, fibrocartilage formation, new bone formation, tendon healing and cartilage injury. RESULTS AND CONCLUSION: The cell morphology, matrix staining intensity and total scores of the 10 times group were significantly higher than those of the other groups at 6 weeks (P 〈 0.05), but other parameters showed no significant differences among groups (P 〉 0.05). ACL reconstructed with 10 times of repetitive freeze-thawing allogenic Achilles tendons had higher histological scores at 6 weeks after modeling, but no significant differences were shown at 12 and 24 weeks after modeling. To conclude, our study only testifies better histological scores on the multiple times of freezethawing Achilles tendon than the less times of freeze-thawing Achilles tendon at the early period after operation.

关 键 词:冷冻 组织学 前交叉韧带 组织工程 组织构建 反复冻融 膝关节 同种异体移植物 重建 

分 类 号:R318[医药卫生—生物医学工程]

 

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