牵张成骨模型兔的建立与评估  

Establishment and evaluation of a rabbit model of distraction osteogenesis

作　　者：孙丙银 朱洁英[2] 熊建尉 赵宝祥[3] Sun Bing-yin Zhu Jie-ying Xiong Jian-wei Zhao Bao-xiang(Foshan Jianxiang Hospital, Foshan 528000, Guangdong Province, China Guangzhou Brain Hospital, Guangzhou 510370, Guangdong Province, China Linyi People＇s Hospital, Linyi 276000, Shandong Province, China)

机构地区：[1]佛山健翔医院,广东省佛山市528000 [2]广州市脑科医院,广东省广州市510370 [3]临沂市人民医院,山东省临沂市276000

出　　处：《中国组织工程研究》2017年第16期2576-2581,共6页Chinese Journal of Tissue Engineering Research

基　　金：2014山东省中青年科学家科研奖励基金(BS2014YY026);2017佛山市卫生和计生局医学科研课题(20170161)~~

摘　　要：背景:牵张成骨技术在临床上成为重要的治疗大段骨缺损、骨肿瘤、骨髓炎等的有效方法,但在国内外进行牵张成骨的基础研究中,尚缺乏建立标准的牵张成骨模型。目的:建立兔股骨牵张成骨模型,评估兔股骨牵张成骨模型的成骨效果。方法:取20只雄性新西兰兔,采用新型的兔股骨牵张成骨器,建立兔股骨牵张成骨动物模型并通过标本一般形态和X射线片观察牵张成骨后的成骨效果。结果与结论:(1)标本一般形态:固定第14天,牵张间隙内新生骨组织颜色变浅,较前致密、匀称,呈圆柱状贯通骨折两端,与正常骨两端界限较前模糊;固定第35天,牵张间隙内可见新生骨组织表面与正常骨颜色、纹理相同,肉眼较难以分清正常骨组织和牵张间隙新生骨区的界限,骨密度明显增高。(2)X射线片检测结果:固定第14天,牵张支架固定稳定,骨折两端对线可,牵张间隙内可见连接正常骨两端的云雾状阴影,密度进一步增高;固定第35天,牵张支架固定稳定,骨折两端对线可,新生骨组织钙化完全,骨皮质完全形成,髓腔畅通;(3)结果证实:实验将自行研制的单臂式牵张支架应用于兔股骨牵张成骨实验,通过设计合理的建模步骤及规范的术后牵张成骨操作,成功建立了兔股骨牵张成骨模型。BACKGROUND： Distraction osteogenesis is an effective method for large bone defects, bone tumors and osteomyelitis. However, there is a lack of a standard model in the basic research concerning distraction osteogenesis. OBJECTIVE： To establish a rabbit femoral model of distraction osteogenesis and to assess its osteogenic effect. METHODS： Twenty male New Zealand rabbits were enrolled to establish the rabbit femoral model of distraction osteogenesis using a novel distractor. Subsequently, the gross observation and X-ray examination of the specimens were performed to assess the osteogenic effect. RESULTS AND CONCLUSION： Gross observation： on day 14 after fixation, there were light-colored and dense newborn bones distributing evenly in the distraction gap, and appeared with a columnar connection with the broken ends; the boundary with normal bones became vague. On day 35, the surface of newborn bones in the distraction gap showed the same color and texture with the normal ones, the boundary between the newborn and normal bones was difficult to distinguish, and the bone mineral density was increased notably. Radiology results： on day 14 after fixation, the stent was fixed stably, the broken ends got good reduction, and cloudy shallows connecting the two ends of the normal bones in the distraction gap and increased bone mineral density were detectable. Completely calcified new-born bones, intact bone cortex and open medullary canal were further visible on day 35. These results suggest that the rabbit femoral model of distraction osteogenesis is established successfully using the self-designed single-arm distractor based on rational surgical procedures and standard operations.

关 键 词：组织工程 模型 动物 股骨 组织构建 骨组织工程 牵张成骨 新西兰兔 模型 牵张支架 建立 评估 大体标本 X射线片 

分 类 号：R318[医药卫生—生物医学工程]