三维打印制备不同大孔形态含锶介孔生物玻璃支架的体外细胞实验  

Three-dimensional printing of strontium-containing mesoporous bioactive glass scaffolds with varied macropore morphologies: an in vitro cytological experiment

作　　者：张旭[1] 吴良浩[1] 李得见 敖荣广[1] 陈帆成 俞斌[1] 禹宝庆[1] Zhang Xu Wu Liang-hao Li De-jian Ao Rong-guang Chen Fan-cheng Yu Bin Yu Bao-qing(Department of Orthopedics, Shanghai Pudong Hospital, Fudan University, Shanghai 200120, China)

机构地区：[1]复旦大学附属浦东医院骨科,上海市200120

出　　处：《中国组织工程研究》2017年第18期2858-2863,共6页Chinese Journal of Tissue Engineering Research

基　　金：上海市科委基础研究领域项目(13JC1407302);浦东新区卫生系统重点学科群建设资助项目(PWZxq2014-03);上海市卫生局重点课题(20134039)~~

摘　　要：背景:三维打印支架的大孔孔径形态对生物组织工程支架理化性能及生物性能有很大的影响。目的:利用三维打印技术制备不同大孔结构的含锶介孔生物玻璃支架,探讨其对MC3T3-E1细胞增殖和成骨分化的影响,寻找最佳的大孔形态。方法:利用三维打印技术制备含锶介孔生物玻璃/聚己内酯复合支架,其中纵横交错的各纤维状经纬线之间的夹角设置为45°,60°和90°,根据其角度命名为45°,60°,90°含锶介孔生物玻璃支架,检测MC3T3-E1细胞在复合支架上的增殖能力和碱性磷酸酶活性。结果与结论:(1)CCK-8方法检测结果表明,3组支架材料都能够支持细胞增殖,第1天和第4天45°含锶介孔生物玻璃支架上的细胞数量略高于60°和90°含锶介孔生物玻璃支架,但差异无显著性意义(P>0.05);第7天45°含锶介孔生物玻璃支架上的细胞数量显著高于60°和90°含锶介孔生物玻璃支架,差异有显著性意义(P<0.05);(2)在第14天,45°含锶介孔生物玻璃支架的细胞碱性磷酸酶活性显著高于60°和90°含锶介孔生物玻璃支架,差异有显著性意意义(P<0.05);在第21天,3组支架的碱性磷酸酶活性差异无显著性意义(P>0.05);(3)结果表明,45°含锶介孔生物玻璃支架相较于60°和90°含锶介孔生物玻璃支架,更能促进MC3T3-E1细胞的增殖和成骨分化。BACKGROUND： Macropore morphology of a composite scaffold prepared by the three-dimensional printing technique is of great importance in determining the physicochemical and biological properties of tissue engineering scaffolds. OBJECTIVE： To fabricate strontium-containing mesoporous（Sr-MBG） bioactive glass（PCL） scaffolds by the three-dimensional printing technique, and to explore the effect of these scaffolds on MC3T3-E1 proliferation and osteogenic differentiation, thereby to find out the optimal macropore morphology. METHODS： Sr-MBG/PCL composite scaffolds were fabricated by the three-dimensional printing technique. The angles between fibrous latitudes and longitudes were set to 45°, 60° and 90°. Then the proliferation and alkaline phosphatase activity of MC3T3-E1 cells on the scaffolds were tested. RESULTS AND CONCLUSION： Cell counting kit-8 results showed that MC3T3-E1 cells could proliferate on all the three kinds of scaffolds. The proliferation rate of MC3T3-E1 cells on the 45° Sr-MBG/PCL scaffolds was just slightly higher than that on the 60° and 90° Sr-MBG/PCL scaffolds at days 1 and 4（P〈0.05）, but there was a significant increase at day 7（P〈0.05）. The 45° Sr-MBG/PCL scaffolds exhibited a significant increase in alkaline phosphatase activity of MC3T3-E1 cells compared to the 60° and 90° Sr-MBG/PCL scaffolds at day 14（P〈0.05）, while there was no significant difference among three groups at day 21（P〈0.05）. These results indicate that the 45° Sr-MBG/PCL scaffold is more suitable to promote the proliferation and osteogenic differentiation of the MC3T3 cells than the 60° and 90° Sr-MBG/PCL scaffolds.

关 键 词：生物材料 材料相容性 三维打印 生物相容性 含锶介孔生物玻璃 聚己内酯 MC3T3-E1细胞 支架 大孔形态 CCK-8 碱性磷酸酶 

分 类 号：R318[医药卫生—生物医学工程]