新型组织工程化改性胶原-壳聚糖复合支架制备与神经细胞的相容性  

A novel tissue-engineered crosslinked collagen-chitosan scaffold and its biocompatibility with nerve cells

作　　者：陆江[1,2,3] 陈绚丽[4] 唐核心[1,2] 佘纳 朱垚[1] 李思星 王天颖[1] 张国辉[1] Lu Jiang Chen Xuan-li Tang He-xin She Na Zhu Yao Li Si-xing Wang Tian-ying Zhang Guo-hui(First Clinical College Institute of Anesthesiology, 4Basic Medical Institute, Hubei University of Medicine,＇ Shiyan 442000, Hubei Province, China Department of Anesthesiology, Affiliated Taihe Hospital of Hubei University of Medicine, Shiyan 442000, Hubei Province, China)

机构地区：[1]湖北医药学院第一临床学院,湖北省十堰市442000 [2]湖北医药学院麻醉学研究所,湖北省十堰市442000 [3]湖北医药学院附属太和医院麻醉科,湖北省十堰市442000 [4]湖北医药学院基础医学研究所,湖北省十堰市442000

出　　处：《中国组织工程研究》2017年第18期2913-2919,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家教育部2012年度国家级大学生创新创业训练计划项目(201210929004);国家教育部2016年度国家级大学生创新创业训练计划项目(201610929005);十堰市科学技术研究与开发项目(14Y13);2016年度湖北医药学院教学研究项目(2016027);2016年度湖北医药学院附属太和医院科学研究基金(2016JJXM001);湖北医药学院高层次人才科研启动金(2011QDZR-1;2011QDZR-2);湖北医药学院2015年度"十二五规划"学科建设学科英才成长资助项目(2015)~~

摘　　要：背景:周围神经缺损的治疗在再生医学领域仍然具有挑战性,支架材料的应用被认为是有前途的探索方向之一,理想的支架材料应是安全、耐用和缺乏抗原性的。目的:探讨制备一种新型的组织工程化改性胶原-壳聚糖支架,并探索其在大鼠横断坐骨神经模型中用作桥梁修复神经损伤的可能。方法:先制备出胶原-壳聚糖复合支架,然后用1-乙基-3-(3-二甲基氨基丙基)-碳化二亚胺,N-羟基琥珀酰亚胺对支架进行化学改性即交联。接着将神经干细胞种植于支架上,之后连同支架-种子细胞在体内植入坐骨神经缺损部位。结果与结论:制得的变性交联胶原-壳聚糖复合支架具有三维立体网状结构。将支架-种子细胞移植4周后,可在损伤的坐骨神经中发现S-100神经丝蛋白200表达。结果表明这种组织工程化改性交联胶原-壳聚糖复合支架具有良好的细胞相容性。BACKGROUND： Treatment for peripheral nerve injury is still a challenge in the field of regenerative medicine. The use of scaffold materials is considered to be one of the promising opportunities to settle these defects. The ideal scaffold material should be safe and durable, but have no antigenicity.OBJECTIVE： To develop a novel tissue-engineered collagen-chitosan composite scaffolds under modification in attempt to repair sciatic nerve injury in rats. METHODS： First the collagen-chitosan composite scaffold was prepared, and then cross-linked with 1-ethyl-3-（3-2 methyl amino propyl）-WC 2 imide（EDC） and N-hydroxy succinimide（NHS）. After modification, nerve cells were seeded onto the scaffold. Finally, the cell-scaffold composite was implanted into the injured sciatic nerve. RESULTS AND CONCLUSION： The EDC/NHS-crosslinked collagen-chitosan scaffold showed a three-dimensional reticular structure. Four weeks after the cell-scaffold composite implantation, neurofilament protein 200 was found to express in the injured sciatic nerve. This indicates that the crosslinked collagen-chitosan scaffold has good cytocompatibility.

关 键 词：生物材料 材料相容性 胶原 壳聚糖 支架 改性 超微结构 孔隙率 降解速率 

分 类 号：R318[医药卫生—生物医学工程]