Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells  

作　　者：Jiang Lu Xue-qin Yao Xin Luo Yu Wang Sookja Kim Chung He-xin Tang Chi Wai Cheung Xian-yu Wang Chen Meng Qing Li 

机构地区：[1]Anesthesiology Research Institute of Hubei University of Medicine [2]Department of Anesthesiology, Taihe Hospital, Hubei University of Medicine [3]Department of Anesthesiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong [4]Laboratory and Clinical Research Institute for Pain, Li Ka Shing Faculty of Medicine, The University of Hong Kong [5]Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong

出　　处：《Neural Regeneration Research》2017年第6期945-952,共8页中国神经再生研究（英文版）

基　　金：supported by the Natural Science Foundation of Hubei Province of China,No.2012FFC060;the Natural Science Foundation of Hubei University of Medicine of China,No.2011QDZR-2;a grant from the Scientific and Technological Project of Shiyan City of Hubei Province of China,No.14Y13;a grant from the National College Student Innovation and Entrepreneurship Training Program of Education Ministry of China,No.201610929005 and No.201210929004;the Educational Scientific Foundation of Hubei University of Medicine of China in 2016,No.2016027;the Fund of Disciplines Construction of Hubei University of Medicine of China in 2015,No.2015;the Scientific Foundation of Affiliated Taihe Hospital of Hubei University of Medicine of China in 2016,No.2016JJXM001

摘　　要：Monosialoganglioside 1（GM1） is the main ganglioside subtype and has neuroprotective properties in the central nervous system. In this study, we aimed to determine whether GM1 alleviates neurotoxicity induced by moderate and high concentrations of propofol combined with remifentanil in the immature central nervous system. Hippocampal neural stem cells were isolated from newborn Sprague-Dawley rats and treated with remifentanil（5, 10, 20 ng/m L） and propofol（1.0, 2.5, 5.0 μg/m L）, and/or GM1（12.5, 25, 50 μg/m L）. GM1 reversed combined propofol and remifentanil-induced decreases in the percentage of 5-bromodeoxyuridine（＋） cells and also reversed the increase in apoptotic cell percentage during neural stem cell proliferation and differentiation. However, GM1 with combined propofol and remifentanil did not affect β-tubulin（＋） or glial fibrillary acidic protein（＋） cell percentage during neural stem cell differentiation. In conclusion, we show that GM1 alleviates the damaging effects of propofol combined with remifentanil at moderate and high exposure concentrations in neural stem cells in vitro, and exerts protective effects on the immature central nervous system.Monosialoganglioside 1（GM1） is the main ganglioside subtype and has neuroprotective properties in the central nervous system. In this study, we aimed to determine whether GM1 alleviates neurotoxicity induced by moderate and high concentrations of propofol combined with remifentanil in the immature central nervous system. Hippocampal neural stem cells were isolated from newborn Sprague-Dawley rats and treated with remifentanil（5, 10, 20 ng/m L） and propofol（1.0, 2.5, 5.0 μg/m L）, and/or GM1（12.5, 25, 50 μg/m L）. GM1 reversed combined propofol and remifentanil-induced decreases in the percentage of 5-bromodeoxyuridine（＋） cells and also reversed the increase in apoptotic cell percentage during neural stem cell proliferation and differentiation. However, GM1 with combined propofol and remifentanil did not affect β-tubulin（＋） or glial fibrillary acidic protein（＋） cell percentage during neural stem cell differentiation. In conclusion, we show that GM1 alleviates the damaging effects of propofol combined with remifentanil at moderate and high exposure concentrations in neural stem cells in vitro, and exerts protective effects on the immature central nervous system.

关 键 词：propofol alleviate tubulin anesthesia moderate newborn protective fibrillary glial aimed 

分 类 号：R614[医药卫生—麻醉学]