内质网应激在大鼠缺血再灌注损伤心肌中的表达  

Express of endoplasmic reticulum stress in rat myocardium with ischemia-reperfusion injury

作　　者：张腾芳[1] 谢晓勇[1] 郑宝石[1] 何巍[1] 罗程[1] 黎玉贵 冯旭[1] ZHANG Tengfang XIE Xiaoyong ZHENG Baoshi HE Wei LUO Cheng LI Yugui FENG Xu(Department of Cardiothoracic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, China)

机构地区：[1]广西医科大学第一附属医院心胸外科,南宁市530021

出　　处：《微创医学》2017年第3期306-308,311,共4页Journal of Minimally Invasive Medicine

基　　金：广西自然科学基金(编号:2013GXNSFAA019152);广西壮族自治区卫生厅课题(编号:Z2012108)

摘　　要：目的探讨内质网应激(ERS)在大鼠缺血再灌注损伤心肌中的表达以及小剂量衣霉素(TM)预处理对缺血再灌注损伤心肌的影响。方法 SD大鼠30只随机分为三组,假手术组(Sham组)、缺血再灌注组(IR组)、衣霉素处理+IR组(TM+IR组),每组10只。Sham组单纯开胸,不结扎冠状动脉;IR组开胸,结扎前降支30 min,再灌注2 h;TM+IR组TM腹腔注射0.6 mg/kg,30 min后结扎前降支30 min,再灌注2 h。于开胸后,心肌再灌注2 h抽血,检测肌钙蛋白I(c Tn I)含量,透射电镜观察再灌注2 h心肌的超微结构,检测再灌注2 h心肌的GRP78 mRNA及蛋白表达。结果 IR组和TM+IR组在再灌注2 h时,c Tn I水平均明显上升;IR组心肌超微结构损伤最严重,TM+IR组次之。再灌注2 h后,GRP78 mRNA及蛋白,IR组及TM+IR组明显升高。结论 MIRI可诱导ERS,小剂量TM预处理可减轻MIRI。Objective To explore the expression of endoplasmic reticulum stress （ERS） in rat myocardium with ischemia-reperfusion injury and the effect of preconditioning with small-dose tunicamycin on myoeardium with isehemia-reperfusion injury. Methods Thirty SD rats were randomly divided into 3 groups including sham-operation group （Sham group ）, ischemia-reperfusion injury group （IR group ） and tunicamyein ＋ ischemia-reperfusion injury （ TM ＋ IR group）, ten rats in each group. Simple thoracotomy without coronary artery ligation was performed in the Sham group. Thoracotomy and two-hour reperfusion after 30 minutes of the anterior descending coronary artery ligation were performed in the IR group. In the IR ＋ TM group, ligation of the anterior descending coronary artery for 30 minutes was performed after 30 minutes of intraperitoneal injection with tunicamyein （0.6ml/kg）, then two-hour reperfusion was conducted. The content of cardiac troponin I （cTnI） was detected after thoracotomy and after 2 hours of cardiac reperfusion. The myocardial ultrastructure was observed under transmission electron microscope and the expressions of myocardial glucose regulated protein 78 （GRP78） mRNA and protein were detected after 2 hours of reperfusion. Results The cTnI levels were significantly increased in the IR group and TM ＋ IR group at 2 hours after reperfusion. The damage of myocardial ultrastructure was the most severe in the IR group , and was the second severe in the TM ＋ IR group. GRP78 mRNA and protein expressions increased significantly in the IR group and TM ＋ IR group at 2 hours after reperfusion. Conclusion Myocardial isehemia-reperfusion injury （MIRI） may induce ERS. And preconditioning with small-dose tunieamyein may alleviate MIRI.

关 键 词：心肌缺血再灌注损伤 内质网应激 衣霉素 葡萄糖调节蛋白78 

分 类 号：R54[医药卫生—心血管疾病]